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          • Primary Antibodies ›
          • Her-2 (ErbB2) Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          ErbB2 (HER-2) Monoclonal Antibody (N12), Biotin

          12 References
          View all (317) Her-2 (ErbB2) antibodies

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          Cite ErbB2 (HER-2) Monoclonal Antibody (N12), Biotin

          Additional Information:
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          • Antibody Testing Data (2)
          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          ErbB2 (HER-2) Antibody (MA5-12995) in ICC/IF

          Immunofluorescent analysis of HER-2 (green) showing staining in the membrane of SK-BR-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a HER-2 monoclonal antibody (Product # MA5-12998) in 3% BSA-PBS at a d... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          ErbB2 (HER-2) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ErbB2 (HER-2) Monoclonal Antibody (N12), Biotin

          Product Details

          MA5-12995

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:200
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 3 publications 3 publications

          Immunomicroscopy (IM)

          Assay-dependent
          -
          Product Specifications

          Published species

          Hamster, Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          N12

          Immunogen

          Intact SKBR-3 breast cancer cells
          3D Epitope / Immunogen

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Amount

          100 µg

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10983436

          Product Specific Information

          MA5-12995 targets HER-2 in ICC/IF, IHC (F), IHC (P) and IM applications and shows reactivity with Human samples.

          The MA5-12995 immunogen is intact SKBR-3 breast cancer cells.

          Target Information

          ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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