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Invitrogen
ErbB4 Monoclonal Antibody (H4.72.8 (Ab72))
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FIGURE: 1 / 7
ErbB4 Antibody (MA5-13016) in WB
Western blot analysis of HER-4 was performed by loading 25 µg of mouse brain cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a HER-4 monoclonal antibody (Product # MA5-13016) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results ... View More
Product Details
MA5-13016
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Ferret,
Human,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
H4.72.8 (Ab72)
Immunogen
Extracellular domain of recombinant human c-erbB-4/HER-4 oncoprotein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13016 targets HER-4 in WB, FACS applications and shows reactivity with Human and Mouse samples.
The MA5-13016 immunogen is extracellular domain of recombinant human c-erbB-4/HER-4 oncoprotein.
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4
UniProt ID: (Human) Q15303, (Mouse) Q61527
Entrez Gene ID: (Human) 2066, (Mouse) 13869
nucleotide binding
transcription regulatory region sequence-specific DNA binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
epidermal growth factor-activated receptor activity
epidermal growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
neuregulin receptor activity
protein homodimerization activity
GABA receptor binding
receptor signaling protein tyrosine kinase activity
transferase activity, transferring phosphorus-containing groups
transcription regulatory region DNA binding
transmembrane signal receptor
neural crest cell migration
positive regulation of protein phosphorylation
apoptotic process
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
JAK-STAT cascade
nervous system development
synapse assembly
heart development
lactation
positive regulation of cell proliferation
negative regulation of cell proliferation
embryonic pattern specification
positive regulation of biosynthetic process
cell migration
peptidyl-tyrosine phosphorylation
central nervous system morphogenesis
olfactory bulb interneuron differentiation
neuron differentiation
regulation of cell migration
mammary gland development
ERBB4 signaling pathway
ERBB2-ERBB4 signaling pathway
ERBB4-ERBB4 signaling pathway
positive regulation of tyrosine phosphorylation of STAT protein
negative regulation of apoptotic process
positive regulation of MAPK cascade
mitochondrial fragmentation involved in apoptotic process
cell fate commitment
positive regulation of transcription, DNA-templated
positive regulation of phosphate metabolic process
positive regulation of JAK-STAT cascade
protein autophosphorylation
positive regulation of epithelial cell proliferation
positive regulation of protein kinase B signaling
positive regulation of cardiac muscle cell proliferation
mammary gland epithelial cell differentiation
mammary gland alveolus development
cardiac muscle tissue regeneration
regulation of biological quality
positive regulation of ERK1 and ERK2 cascade
cellular response to epidermal growth factor stimulus
establishment of planar polarity involved in nephron morphogenesis
neurotransmitter receptor localization to postsynaptic specialization membrane
positive regulation of protein localization to cell surface
negative regulation of neuron migration
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
multicellular organism development
negative regulation of muscle cell apoptotic process
phosphorylation
positive regulation of cell migration
positive regulation of synaptic transmission, GABAergic
positive regulation of tyrosine phosphorylation of Stat5 protein
surfactant homeostasis
positive regulation of glucose import
synapse maturation
positive regulation of STAT protein import into nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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