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Invitrogen
Ku70 Monoclonal Antibody (N3H10)
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FIGURE: 1 / 19
Ku70 Antibody (MA5-13110) in ICC/IF
Immunofluorescence analysis of Ku70 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ku70 Monoclonal Antibody (N3H10) (Product # MA5-13110) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More
Product Details
MA5-13110
Applications
Tested Dilution
Publications
Product Specifications
Published species
Hamster,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N3H10
Immunogen
Human placental extract designated as PSE1-PL
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-13110 targets Ku (p70) in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, FACS and Western blot applications and shows reactivity with Human, Non-human primate, and Xenopus laevis samples. Reacts with mouse and hamster in Western blot applications only.
The MA5-13110 immunogen is human placental extract designated as PSE1-PL.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Target Information
XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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