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Invitrogen
MGMT Monoclonal Antibody (MT 3.1)
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FIGURE: 1 / 11
MGMT Antibody (MA5-13506) in ICC/IF
Immunofluorescence analysis of Methylated-DNA-protein-cysteine methyltransferase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MGMT Monoclonal Antibody (MT 3.1) (Product # MA5-13506) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Seconda... View More
Product Details
MA5-13506
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MT 3.1
Immunogen
Purified recombinant human MGMT protein
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Amount
200 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-13506 targets MGMT (O-methylguanine-DNA methyltransferase) in FACS, ICC/IF, IHC (P), and WB applications and shows reactivity with Human samples.
The MA5-13506 immunogen is purified recombinant human MGMT protein.
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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