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ErbB2 (HER-2) Monoclonal Antibody (3B5), Biotin
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FIGURE: 1 / 5
ErbB2 (HER-2) Antibody (MA5-13672) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (1) and MDA-MB-231 (2). The blots were probed with Anti-ErbB2 Mouse Monoclonal Antibody (Product # MA5-13672, 1 µg/mL dilution) and detected by chemiluminescence using Streptavidin-HRP (Product # N200, 1:10,000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 but not in MDA-MB-231, which is a negative control for ErbB2. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-13672
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Avian,
Cat,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3B5
Immunogen
A synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13672 targets HER-2 in IP and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-13672 immunogen is a synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.
Target Information
ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: approximately 68 kDa secreted autoinhibitor glycoprotein; avian erythroblastosis oncogene B 2; c-erb B2/neu protein; c-erbB-2; CD antigen CD340; erb-b2; erbb 2; erbB2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; HER2 receptor; HER2/neu receptor; herstatin; human epidermal growth factor receptor 2; metas; metastatic lymph node gene 19 protein; Neu oncogene; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; proto-oncogene c-ErbB-2; proto-oncogene NEU; tyrosine kinase-type cell surface receptor HER2; unnamed protein product; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
Gene Aliases: c-ERB-2; c-ERB2; c-erbB2; c-neu; CD340; Erbb-2; HER-2; HER-2/neu; HER2; l11Jus8; mKIAA3023; MLN 19; MLN-19; NEU; NGL; p185(erbB2); TKR1; VSCN2
UniProt ID: (Human) P04626, (Mouse) P70424
Entrez Gene ID: (Human) 2064, (Mouse) 13866
RNA polymerase I core binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
transmembrane signaling receptor activity
receptor binding
protein binding
ATP binding
coreceptor activity
growth factor binding
receptor tyrosine kinase binding
identical protein binding
ErbB-3 class receptor binding
protein heterodimerization activity
ubiquitin protein ligase binding
macromolecular complex binding
Hsp90 protein binding
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
nervous system development
peripheral nervous system development
heart development
neuromuscular junction development
motor neuron axon guidance
cell proliferation
positive regulation of cell proliferation
glial cell differentiation
positive regulation of gene expression
Schwann cell development
peptidyl-tyrosine phosphorylation
neuron differentiation
positive regulation of cell growth
axon regeneration
regulation of microtubule-based process
negative regulation of immature T cell proliferation in thymus
positive regulation of Rho protein signal transduction
intracellular signal transduction
ERBB signaling pathway
ERBB2-ERBB3 signaling pathway
ERBB2-EGFR signaling pathway
ERBB2-ERBB4 signaling pathway
wound healing
regulation of cell proliferation
myelination
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
protein kinase B signaling
estrous cycle
positive regulation of translation
positive regulation of cell adhesion
positive regulation of transcription from RNA polymerase I promoter
positive regulation of JNK cascade
positive regulation of Ras protein signal transduction
protein autophosphorylation
sympathetic nervous system development
animal organ development
response to axon injury
oligodendrocyte differentiation
positive regulation of epithelial cell proliferation
positive regulation of small GTPase mediated signal transduction
positive regulation of protein kinase B signaling
regulation of ERK1 and ERK2 cascade
cellular response to growth factor stimulus
cellular response to epidermal growth factor stimulus
semaphorin-plexin signaling pathway
positive regulation of protein targeting to membrane
neurotransmitter receptor localization to postsynaptic specialization membrane
positive regulation of membrane permeability
positive regulation of neural precursor cell proliferation
protein phosphorylation
enzyme linked receptor protein signaling pathway
ERBB2 signaling pathway
regulation of angiogenesis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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