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Invitrogen
Cyclin B1 Monoclonal Antibody (GNS11)
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FIGURE: 1 / 11
Cyclin B1 Antibody (MA5-14327) in ICC/IF
Immunofluorescent analysis of Cyclin B1 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Cyclin B1 monoclonal antibody (Produc... View More
Product Details
MA5-14327
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GNS11
Immunogen
Human cyclin B1 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-14327 targets Cyclin B1 in WB, ICC/IF, IHC (P), and IP applications and shows reactivity with Hamster, Human, mouse, and Rat samples. This antibody is not recommended for NIH-3T3 cells in Western blot applications.
The MA5-14327 immunogen is human cyclin B1 protein.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Target Information
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023; unnamed protein product
Gene Aliases: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1; I79_013589
UniProt ID: (Human) P14635, (Chinese hamster) Q08301, (Rat) P30277, (Mouse) P24860
Entrez Gene ID: (Human) 891, (Chinese hamster) 100770842, (Rat) 25203, (Mouse) 268697
patched binding
protein binding
cyclin-dependent protein serine/threonine kinase regulator activity
protein kinase binding
ubiquitin-like protein ligase binding
cyclin-dependent protein serine/threonine kinase activator activity
kinase activity
protein complex binding
histone kinase activity
kinase activator
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
oocyte maturation
in utero embryonic development
mitotic spindle organization
mitotic metaphase plate congression
spermatogenesis
response to xenobiotic stimulus
response to mechanical stimulus
response to toxic substance
negative regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of mRNA 3'-end processing
tissue regeneration
mitotic cell cycle phase transition
positive regulation of cell cycle
positive regulation of mitotic cell cycle
response to DDT
positive regulation of fibroblast proliferation
digestive tract development
cell division
positive regulation of attachment of spindle microtubules to kinetochore
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
regulation of chromosome condensation
protein-containing complex assembly
cellular response to iron(III) ion
cellular response to fatty acid
cellular response to hypoxia
regulation of mitotic cell cycle spindle assembly checkpoint
positive regulation of mitochondrial ATP synthesis coupled electron transport
negative regulation of protein phosphorylation
protein complex assembly
protein phosphorylation
cell cycle
mitotic nuclear division
positive regulation of histone phosphorylation
histone H3-S10 phosphorylation involved in chromosome condensation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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