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Invitrogen
RAD51 Monoclonal Antibody (51RAD01 (3C10))
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FIGURE: 1 / 13
RAD51 Antibody (MA5-14419) in ICC/IF
Immunofluorescent analysis of RAD51 (green) showing staining in the cytoplasm and nucleus of H1299 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a RAD51 monoclonal antibody (Product # MA5-14419) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyL... View More
Product Details
MA5-14419
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Algae,
Fish,
Human,
Mouse,
Pig,
Protozoa,
Yeast
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
51RAD01 (3C10)
Immunogen
Recombinant Rad51 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-14419 targets RAD51 in IHC (P), IP, ICC/IF and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-14419 immunogen is recombinant Rad51 protein.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51; RAD51 homolog; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A; unnamed protein product
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603
UniProt ID: (Human) Q06609, (Mouse) Q08297
Entrez Gene ID: (Human) 5888, (Mouse) 19361, (Rat) 499870
recombinase activity
nucleotide binding
DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
protein binding
ATP binding
DNA-dependent ATPase activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
enzyme binding
identical protein binding
DNA polymerase binding
ATP-dependent DNA damage sensor activity
damaged DNA binding
protein C-terminus binding
single-stranded DNA-dependent ATPase activity
molecular_function
DNA metabolism protein
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA metabolic process
DNA repair
DNA recombination
mitotic recombination
cellular response to DNA damage stimulus
meiosis I
reciprocal meiotic recombination
response to xenobiotic stimulus
response to toxic substance
response to X-ray
regulation of double-strand break repair via homologous recombination
telomere maintenance via telomere lengthening
replication fork processing
telomere organization
interstrand cross-link repair
strand invasion
meiotic cell cycle
chromosome organization involved in meiotic cell cycle
cellular response to alkaloid
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to hydroxyurea
cellular response to cisplatin
cellular response to camptothecin
response to glucoside
replication-born double-strand break repair via sister chromatid exchange
mitotic recombination-dependent replication fork processing
double-strand break repair involved in meiotic recombination
regulation of DNA damage checkpoint
regulation of protein phosphorylation
DNA unwinding involved in DNA replication
meiotic nuclear division
positive regulation of DNA ligation
protein homooligomerization
response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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