Search

  • Contact Us
  • Quick Order

Invitrogen

IRE1 alpha Monoclonal Antibody (J.607.10)

Advanced Verification
1 Published Figure
1 Reference
View all (50) IRE1 alpha antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
IRE1 alpha Monoclonal Antibody (J.607.10)

Product Details

MA5-14991

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
View 1 publication 1 publication

Immunoprecipitation (IP)

1:50
-

RNA Immunoprecipitation (RIP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

J.607.10

Immunogen

Synthetic peptide corresponding to residues surrounding His963 of human IRE1a
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

108 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977010

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ern1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; Ire1p; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; unnamed protein product

View more View less

Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P

View more View less

UniProt ID: (Human) O75460, (Mouse) Q9EQY0

View more View less

Entrez Gene ID: (Human) 2081, (Mouse) 78943

View more View less

Function(s)
nucleotide binding magnesium ion binding catalytic activity endonuclease activity endoribonuclease activity ribonuclease activity protein kinase activity protein serine/threonine kinase activity platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity hydrolase activity enzyme binding Hsp70 protein binding identical protein binding protein homodimerization activity ADP binding metal ion binding unfolded protein binding Hsp90 protein binding protein serine kinase activity bidentate ribonuclease III activity transferase activity, transferring phosphorus-containing groups endoribonuclease
Process(es)
endothelial cell proliferation mRNA processing RNA catabolic process mRNA catabolic process protein phosphorylation apoptotic process response to unfolded protein negative regulation of gene expression regulation of macroautophagy endoplasmic reticulum unfolded protein response positive regulation of RNA splicing cellular response to unfolded protein response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus IRE1-mediated unfolded protein response positive regulation of JNK cascade mRNA splicing, via endonucleolytic cleavage and ligation intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to glucose stimulus positive regulation of endoplasmic reticulum unfolded protein response insulin metabolic process positive regulation of vascular smooth muscle cell proliferation positive regulation of response to endoplasmic reticulum stress transcription, DNA-templated regulation of transcription, DNA-templated mRNA cleavage activation of signaling protein activity involved in unfolded protein response cell cycle arrest activation of JUN kinase activity metabolic process phosphorylation mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response peptidyl-serine autophosphorylation protein autophosphorylation mRNA endonucleolytic cleavage involved in unfolded protein response RNA phosphodiester bond hydrolysis, endonucleolytic peptidyl-serine trans-autophosphorylation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support