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Invitrogen
IRE1 alpha Monoclonal Antibody (J.607.10)
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FIGURE: 1 / 6
IRE1 alpha Antibody (MA5-14991) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), PANC-1 (Lane 2), Raji (Lane 3), Neuro-2a (Lane 4) and HEK293T (Lane 5). The blot was probed with Anti-IRE1 alpha Monoclonal Antibody (J.607.10) (Product # MA5-14991, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 120 kDa band corresponding to IRE1 alpha was observed in all the cell lines tested.
Product Details
MA5-14991
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
J.607.10
Immunogen
Synthetic peptide corresponding to residues surrounding His963 of human IRE1a
Conjugate
Form
Liquid
Concentration
108 µg/mL
Amount
10.8 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ern1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; Ire1p; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; unnamed protein product
Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P
UniProt ID: (Human) O75460, (Mouse) Q9EQY0
Entrez Gene ID: (Human) 2081, (Mouse) 78943
nucleotide binding
magnesium ion binding
catalytic activity
endonuclease activity
endoribonuclease activity
ribonuclease activity
protein kinase activity
protein serine/threonine kinase activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
hydrolase activity
enzyme binding
Hsp70 protein binding
identical protein binding
protein homodimerization activity
ADP binding
metal ion binding
unfolded protein binding
Hsp90 protein binding
protein serine kinase activity
bidentate ribonuclease III activity
transferase activity, transferring phosphorus-containing groups
endoribonuclease
endothelial cell proliferation
mRNA processing
RNA catabolic process
mRNA catabolic process
protein phosphorylation
apoptotic process
response to unfolded protein
negative regulation of gene expression
regulation of macroautophagy
endoplasmic reticulum unfolded protein response
positive regulation of RNA splicing
cellular response to unfolded protein
response to endoplasmic reticulum stress
cellular response to vascular endothelial growth factor stimulus
IRE1-mediated unfolded protein response
positive regulation of JNK cascade
mRNA splicing, via endonucleolytic cleavage and ligation
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to glucose stimulus
positive regulation of endoplasmic reticulum unfolded protein response
insulin metabolic process
positive regulation of vascular smooth muscle cell proliferation
positive regulation of response to endoplasmic reticulum stress
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA cleavage
activation of signaling protein activity involved in unfolded protein response
cell cycle arrest
activation of JUN kinase activity
metabolic process
phosphorylation
mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response
peptidyl-serine autophosphorylation
protein autophosphorylation
mRNA endonucleolytic cleavage involved in unfolded protein response
RNA phosphodiester bond hydrolysis, endonucleolytic
peptidyl-serine trans-autophosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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