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Invitrogen

LSD1 Monoclonal Antibody (E.289.8)

View all (49) LSD1 antibodies

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Product Details

MA5-15026

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E.289.8

Immunogen

Recombinant human LSD1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11007518

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; Flavin-containingso amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; neuroprotective protein 3; RP1-184J9.1; unnamed protein product

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Gene Aliases: 1810043O07Rik; AA408884; AIMAH3; AOF2; BHC110; CPRF; D4Ertd478e; KDM1; KDM1A; KIAA0601; LSD1; mKIAA0601; RGD1562975

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UniProt ID: (Human) Q96AW4, (Mouse) Q6ZQ88, (Rat) A0A8I6G2K8

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Entrez Gene ID: (Human) 23028, (Mouse) 99982, (Rat) 500569

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Function(s)
p53 binding chromatin binding transcription coactivator activity transcription corepressor activity RNA binding protein binding oxidoreductase activity enzyme binding histone demethylase activity histone demethylase activity (H3-K4 specific) histone demethylase activity (H3-K9 specific) telomeric DNA binding identical protein binding MRF binding flavin adenine dinucleotide binding androgen receptor binding RNA polymerase II sequence-specific DNA binding transcription factor binding telomeric repeat-containing RNA binding DNA-binding transcription factor binding protein demethylase activity FAD-dependent H3K4me/H3K4me3 demethylase activity promoter-specific chromatin binding RNA polymerase II transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding N-ethylmaleimide reductase activity ligand-dependent nuclear receptor transcription coactivator activity demethylase activity histone demethylase activity (H3-dimethyl-K4 specific) reduced coenzyme F420 dehydrogenase activity sulfur oxygenase reductase activity malolactic enzyme activity NADPH:sulfur oxidoreductase activity transcription regulatory region DNA binding epoxyqueuosine reductase activity oxidase
Process(es)
negative regulation of transcription from RNA polymerase II promoter epithelial to mesenchymal transition positive regulation of neuroblast proliferation chromatin organization regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of double-strand break repair via homologous recombination positive regulation of epithelial to mesenchymal transition positive regulation of neuron projection development cerebral cortex development positive regulation of protein ubiquitination regulation of protein localization cellular response to UV protein modification process regulation of gene expression, epigenetic neuron maturation negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of cell differentiation positive regulation of cell size positive regulation of transcription from RNA polymerase II promoter guanine metabolic process regulation of neurogenesis positive regulation of multicellular organismal process muscle cell development regulation of androgen receptor signaling pathway response to fungicide cellular response to cAMP cellular response to gamma radiation positive regulation of cold-induced thermogenesis DNA repair-dependent chromatin remodeling negative regulation of transcription initiation-coupled chromatin remodeling negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of neural precursor cell proliferation positive regulation of stem cell proliferation in utero embryonic development transcription, DNA-templated protein demethylation multicellular organism development cell proliferation regulation of primitive erythrocyte differentiation chromatin modification pituitary gland development granulocyte differentiation negative regulation of protein binding histone H3-K9 demethylation histone H3-K4 demethylation positive regulation of chromatin binding negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of erythrocyte differentiation positive regulation of megakaryocyte differentiation negative regulation of transcription, DNA-templated positive regulation of hormone biosynthetic process positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation oxidation-reduction process negative regulation of transcription from RNA polymerase II promoter by histone modification alternative mRNA splicing, via spliceosome response to organic cyclic compound
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