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Invitrogen
IRS1 Monoclonal Antibody (K.346.8)
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FIGURE: 1 / 6
IRS1 Antibody (MA5-15068) in ICC/IF
Immunofluorescence analysis of Insulin receptor substrate 1 was performed using 70% confluent log phase MIA PaCa-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IRS1 Monoclonal Antibody (K.346.8) (Product # MA5-15068) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More
Product Details
MA5-15068
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Pig
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
K.346.8
Immunogen
Synthetic peptide corresponding to the sequence surrounding Ser307 of human IRS-1
Conjugate
Form
Liquid
Concentration
84 µg/mL
Amount
8.4 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with IRS-2.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM
UniProt ID: (Human) P35568, (Rat) P35570, (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Rat) 25467, (Mouse) 16367
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
receptor signaling complex scaffold activity
signaling adaptor activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
signal transducer activity
protein kinase binding
protein domain specific binding
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of signal transduction
positive regulation of glucose metabolic process
cytokine-mediated signaling pathway
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
glucose homeostasis
protein kinase B signaling
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to fatty acid
positive regulation of mesenchymal cell proliferation
regulation of gene expression
phosphatidylinositol 3-kinase signaling
lipid catabolic process
insulin secretion
positive regulation of cell migration
mammary gland development
response to caffeine
protein localization to nucleus
positive regulation of phosphorylation
response to peptide hormone
positive regulation of phosphatidylinositol 3-kinase activity
protein heterooligomerization
positive regulation of glucagon secretion
negative regulation of somatostatin secretion
positive regulation of glucose import in response to insulin stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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