Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Pipettes and Pipette Tips
    • Lab Centrifuges
    • Ultra-Low Temperature Freezers
    • Spectroscopy
    • Beakers
    • PCR Equipment and Supplies
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • See all product categories
  • Applications
    • Cell Analysis
    • Lab Equipment
    • Real-Time PCR
    • PCR
    • Chromatography
    • Cell Culture and Transfection
    • DNA and RNA Extraction and Analysis
    • Protein Biology
    • Flow Cytometry
    • Chemicals
    • See all applications and techniques
  • Services
    • Custom Services
    • Lab Informatics
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Instrument Services
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • Customer Center
    • Contact Us
    • Certificates of Analysis and Conformance
    • Safety Data Sheets (SDS)
    • Manuals
    • How to Cite Our Products in a Paper
    • Instrument Support
    • Knowledge Base and Product FAQs
    • Learning Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Custom Products & Projects
            • Services Central
          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 650

          1 Published Figure
          29 References
          View all (83) HIF1A antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 650

          Additional Information:

          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Published Figures (1)
          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          HIF1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          HIF1A Antibody (MA5-16008) in ICC/IF

          Redox biology 2019 - Fig. 9 Mitochondrial translocation of endogenous HIF-1alpha in mouse liver after induction of fibrosis. A, B. H&E (A) and Sirius red (B) staining of liver sections from mice administered CCl 4 for 0, 2, 4, or 6 weeks. Scale bars, 100 um. C. Immunofluorescence microscopy of liver sections stained for HIF-1alpha (green) and mitochondria (red, MitoTracker Deep Red) in fibrotic mouse liver. Nuclei were stained ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          HIF1A Antibody in Immunocytochemistry (ICC/IF)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 650

          Product Details

          MA5-16008

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 7 publications 7 publications

          Immunohistochemistry (IHC)

          -
          View 19 publications 19 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-Dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100
          -

          Immunohistochemistry - Free Floating (IHC (Free))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          Assay-Dependent
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          Assay-Dependent
          -

          ELISA (ELISA)

          Assay-Dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          ChIP assay (ChIP)

          Assay-dependent
          -

          Proximity Ligation Assay (PLA) (PLA)

          Assay-dependent
          -

          Gel Shift (EMSA)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Dog, Cat, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus

          Published species

          Human, Mouse, Rabbit, Rat

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          H1alpha67

          Immunogen

          Fusion protein containing amino acids 432-528 of human HIF-1 alpha.
          3D Epitope / Immunogen

          Conjugate

          DyLight™ 650 DyLight™ 650 DyLight™ 650
          • Unconjugated
          • Biotin 
          • DyLight 488
          • DyLight 550
          • Request custom conjugation

          Excitation/Emission Max

          651/673 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.58 mg/mL

          Amount

          58 µg

          Purification

          Protein G

          Storage buffer

          50mM sodium borate

          Contains

          0.05% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11156806

          Product Specific Information

          In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.

          For WB, testing on nuclear extracts is recommended.

          Suggested positive control: Cos-7 nuclear extracts.

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF-1alpha 786; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8; transcription factor; transcription factor; hypoxia-inducible factor 1a; transcription factor; RH1F-1; unnamed protein product

          View more View less

          Gene Aliases: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1a; HIF1alpha; MOP1; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

          View more View less

          UniProt ID: (Human) Q16665, (Rat) O35800, (Mouse) Q61221, (Xenopus) Q9I8A9

          View more View less

          Entrez Gene ID: (Bovine) 281814, (Human) 3091, (Pig) 396696, (Sheep) 443519, (Dog) 480348, (Rabbit) 100009579, (Rat) 29560, (Mouse) 15251, (Xenopus) 100337573, (Xenopus) 445838, (Xenopus) 380141

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding histone acetyltransferase binding sequence-specific DNA binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding histone deacetylase binding protein dimerization activity Hsp90 protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding transcription regulator activator activity macromolecular complex binding basic helix-loop-helix transcription factor
          Process(es)
          response to hypoxia positive regulation of vascular endothelial growth factor production axonal transport of mitochondrion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia response to reactive oxygen species cellular glucose homeostasis epithelial to mesenchymal transition positive regulation of endothelial cell proliferation connective tissue replacement involved in inflammatory response wound healing regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter signal transduction response to iron ion regulation of gene expression vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration negative regulation of ossification positive regulation of vascular endothelial growth factor receptor signaling pathway oxygen homeostasis positive regulation of chemokine production regulation of transforming growth factor beta2 production collagen metabolic process cellular response to oxidative stress positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of glycolytic process positive regulation of hormone biosynthetic process elastin metabolic process positive regulation of chemokine-mediated signaling pathway cellular response to interleukin-1 cellular response to virus positive regulation of cytokine production involved in inflammatory response negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of reactive oxygen species biosynthetic process regulation of protein neddylation negative regulation of transcription from RNA polymerase II promoter angiogenesis blood vessel development neural crest cell migration embryonic placenta development B-1 B cell homeostasis vasculature development heart looping positive regulation of neuroblast proliferation outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process cellular iron ion homeostasis acute-phase response heart development lactation positive regulation of cell proliferation visual learning response to xenobiotic stimulus response to mechanical stimulus response to salt stress response to glucose response to X-ray positive regulation of endothelial cell migration response to auditory stimulus response to purine-containing compound response to activity response to muscle activity positive regulation of macroautophagy neural fold elevation formation cerebral cortex development cell differentiation lung development negative regulation of bone mineralization negative regulation of TOR signaling response to cobalt ion response to estradiol cellular response to insulin stimulus embryonic hemopoiesis positive regulation of vascular wound healing positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process response to alkaloid negative regulation of neuron apoptotic process response to estrogen positive regulation of gluconeogenesis positive regulation of cell size negative regulation of vasoconstriction negative regulation of growth muscle cell cellular homeostasis mitochondrial iron ion transport blood vessel morphogenesis digestive tract morphogenesis camera-type eye morphogenesis positive regulation of smooth muscle cell proliferation tissue remodeling regulation of catalytic activity cartilage development positive regulation of multicellular organismal process response to glucocorticoid maternal process involved in female pregnancy intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development iris morphogenesis retina vasculature development in camera-type eye positive regulation of stress granule assembly regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to estrogen stimulus cellular response to lipid cellular response to anoxia cellular response to xenobiotic stimulus cellular response to light stimulus dopaminergic neuron differentiation response to anesthetic circulatory system development cellular response to toxic substance hypoxia-inducible factor-1alpha signaling pathway regulation of cellular response to hypoxia positive regulation of sprouting angiogenesis cellular response to cyanide cellular response to wortmannin cellular response to phorbol 13-acetate 12-myristate negative regulation of mesenchymal cell apoptotic process tube development animal organ development
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Social Responsibility Social Responsibility
          • Trademarks
          • Fair Trade Fair Trade
          • Policies and Notices
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          Korea flag icon
          Korea

          Customer Help Desk | working day 09:00~18:00
          1661-9555   |   Live Chat   |   카카오톡 상담

           

          Service Call Center | working day 09:00~18:00
          1661-5055   |   Live Chat

          Customer Help Desk | working day 09:00~18:00
          1661-9555   |   Live Chat   |   카카오톡 상담

           

          Service Call Center | working day 09:00~18:00
          1661-5055   |   Live Chat

          Customer Help Desk | working day 09:00~18:00
          1661-9555   |   Live Chat   |   카카오톡 상담

           

          Service Call Center | working day 09:00~18:00
          1661-5055   |   Live Chat

          Thermo Fisher Scientific Korea Ltd.
          Representative : Soojin Seok
          Company Registration No. : 117-81-46910

           

          Thermo Fisher Scientific Solutions LLC
          Representative : Soojin Seok
          Company Registration No. : 114-86-04783

           

          Location: 12F Suseo Office Building, 281 Gwangpyeong-ro, Gangnam-gu, Seoul, Korea(06349) | Mail-Order Business Registration : 2015-Seoul Gangnam-00898 | Payment : ShinHan Bank 140-004-396660 (Thermo Fisher Scientific Solutions LLC)

          ISMS Logo

          Your items have has been added!


          Host server : magellan-search-blue-74d7b4b4d9-zrl6d:80/100.66.77.38:80.
          git-commit: 7aafa54bd143a040505a257d2a3ebda4095a617f
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.56.0-Offline