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FOXP3 Monoclonal Antibody (3G3), APC
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FIGURE: 1 / 4
FOXP3 Antibody (MA5-16224) in Flow
Flow cytometry analysis of FOXP3 in mouse splenocytes. Samples were incubated with FOXP3 monoclonal antibody (Product # MA5-16224) using a dilution of 0.1 µg/10^6 cells. Mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right). Anti-CD25 FITC conjugate this antibody, anti-FOXP3 APC conjugate this antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16224
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3G3
Immunogen
Full-length His-tagged murine FOXP3.
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.67 mg/mL
Amount
67 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Cross reacts with Human and Mouse.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: forkhead box P3; forkhead box P3 transcript variant; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; JM2; MGC141961; MGC141963; RP23-54C14.1; scurfy; unnamed protein product
Gene Aliases: AIID; DIETER; IPEX; JM2; PIDX; scurfin; sf; XPID
UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6
Entrez Gene ID: (Human) 50943, (Mouse) 20371
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription cofactor activity
transcription corepressor activity
protein binding
histone acetyltransferase binding
identical protein binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
NF-kappaB binding
NFAT protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
response to reactive oxygen species
negative regulation of cytokine production
CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment
regulation of T cell anergy
negative regulation of T cell cytokine production
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of cell proliferation
response to virus
establishment of endothelial blood-brain barrier
response to lipopolysaccharide
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
negative regulation of interleukin-2 production
negative regulation of interleukin-4 production
positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation
T cell activation
negative regulation of T cell proliferation
T cell homeostasis
regulatory T cell differentiation
positive regulation of regulatory T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of activated T cell proliferation
negative regulation of defense response to virus
negative regulation of immune response
response to rapamycin
negative regulation of T-helper 17 cell differentiation
B cell homeostasis
myeloid cell homeostasis
T cell mediated immunity
tolerance induction
tolerance induction to self antigen
regulation of immunoglobulin production
positive regulation of T cell tolerance induction
positive regulation of T cell anergy
negative regulation of chronic inflammatory response
positive regulation of peripheral T cell tolerance induction
gene expression
positive regulation of gene expression
negative regulation of gene expression
negative regulation of interleukin-5 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interleukin-4 production
positive regulation of transforming growth factor beta1 production
positive regulation of immature T cell proliferation in thymus
negative regulation of isotype switching to IgE isotypes
regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
T cell receptor signaling pathway
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