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Invitrogen
CD68 Monoclonal Antibody (ED1)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16654
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ED1
Immunogen
Rat spleen cells
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of approximately 90 -110 KDa, also known as rat CD68 (Dijkstra et al. 1985).
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD68 antigen
Entrez Gene ID: (Bovine) 504960, (Rat) 287435
inflammatory response to antigenic stimulus
negative regulation of dendritic cell antigen processing and presentation
cellular response to nutrient levels
response to ethanol
cellular response to lipopolysaccharide
establishment of protein localization to organelle
cellular response to oxidised low-density lipoprotein particle stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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