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Invitrogen

TLR4 Monoclonal Antibody (HTA125)

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View all (71) TLR4 (CD284) antibodies

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Product Details

MA5-16719

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1:10-1:25
View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent
-

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Dog, Guinea pig, Human, Pig, Rhesus monkey

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

HTA125

Immunogen

Ba/F3 cell line expressing TLR4 (CD284)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

200 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538211

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD284; homolog of Drosophila toll; hToll; similar to the Drosophila Toll protein; LRR motif; similar to the cytoplasmic domain of IL-1 receptor; TLR4; toll like receptor 4 protein; Toll-like receptor 4; Toll-like receptor4 protein; toll/interleukin-l receptor (TIR) domain; unnamed protein product

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Gene Aliases: ARMD10; CD284; EGK_07381; hToll; TLR-4; TLR4; TOLL

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UniProt ID: (Human) O00206

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Entrez Gene ID: (Rhesus monkey) 574360, (Pig) 399541, (Human) 7099, (Dog) 403417, (Guinea pig) 100724803

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Function(s)
lipopolysaccharide receptor activity transmembrane signaling receptor activity NAD(P)+ nucleosidase activity NAD+ nucleotidase, cyclic ADP-ribose generating lipopolysaccharide binding beta-amyloid binding receptor binding protein binding signaling receptor activity identical protein binding protein heterodimerization activity
Process(es)
lipopolysaccharide-mediated signaling pathway detection of lipopolysaccharide interleukin-1 beta production innate immune response positive regulation of NLRP3 inflammasome complex assembly activation of innate immune response toll-like receptor signaling pathway wound healing involved in inflammatory response B cell proliferation involved in immune response immune system process nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway nitric oxide biosynthetic process phagocytosis defense response inflammatory response immune response signal transduction I-kappaB kinase/NF-kappaB signaling JNK cascade response to bacterium gene expression positive regulation of platelet activation positive regulation of gene expression astrocyte development microglia differentiation positive regulation of smooth muscle cell migration detection of fungus neurogenesis signal transduction involved in regulation of gene expression positive regulation of B cell proliferation response to lipopolysaccharide regulation of tumor necrosis factor production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade toll-like receptor 4 signaling pathway regulation of toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway cellular response to platelet-derived growth factor stimulus T-helper 1 type immune response B cell proliferation macrophage activation defense response to bacterium positive regulation of macrophage activation positive regulation of I-kappaB kinase/NF-kappaB signaling leukocyte activation MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade lymphocyte proliferation positive regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation regulation of inflammatory response positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NF-kappaB transcription factor activity NK T cell activation stress-activated MAPK cascade detection of stimulus intestinal epithelial structure maintenance positive regulation of macrophage cytokine production ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to mechanical stimulus cellular response to interferon-gamma negative regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of reactive oxygen species biosynthetic process positive regulation of cellular response to macrophage colony-stimulating factor stimulus positive regulation of matrix metallopeptidase secretion cellular response to beta-amyloid positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of extrinsic apoptotic signaling pathway
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