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Invitrogen
CD8 alpha Monoclonal Antibody (MIL12), PE
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Product Details
MA5-16850
Product Specifications
Species Reactivity
Pig
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
MIL12
Immunogen
Porcine mesenteric lymphocytes
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Pig wCD8 alpha antibody, clone MIL12 recognizes an epitope on the alpha chain of porcine wCD8.
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8 antigen alpha polypeptide; CD8 antigen beta polypeptide; CD8A; T-cell surface glycoprotein; CD8alpha; CD8beta; fCD8; Leu-2; leu-2a; T-cell surface glycoprotein
Entrez Gene ID: (Pig) 396627, (Pig) 396636
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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