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Invitrogen
CD31 Monoclonal Antibody (TLD-3A12), FITC
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FIGURE: 1 / 3
CD31 Antibody (MA5-16952) in Flow
Molecular medicine reports 2020 -
Figure 1. Isolation and identification of rat pMSCs. (A) Primary culture and P2 generation cell morphology (magnification, x100). (B) The three on the left panels are unstained negative controls of flow cytometry analysis, while the six on the right are the quantitative analysis of CD44, CD90, CD29, CD31, CD45 and CD11b/c. (C) Immunofluorescence staining of pMSCs. (a and b) Control group. (c and d) Experime... View More
Product Details
MA5-16952
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rhesus monkey,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
TLD-3A12
Immunogen
Activated, Lewis rat derived microglial cells
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule
Gene Aliases: CD31; Pecam; Pecam1
UniProt ID: (Rat) Q3SWT0
Entrez Gene ID: (Rhesus monkey) 718302, (Rat) 29583
positive regulation of leukocyte migration
phagocytosis
cell adhesion
Rho protein signal transduction
regulation of cell migration
negative regulation of actin filament polymerization
response to cytokine
negative regulation of GTPase activity
wound healing
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of peptidyl-tyrosine phosphorylation
diapedesis
glomerular endothelium development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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