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FIGURE: 1 / 10
RhoGDI Antibody (MA5-17032) in ICC/IF
Immunofluorescence analysis of RhoGDI was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RhoGDI Monoclonal Antibody (2G3) (Product # MA5-17032) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product... View More
Product Details
MA5-17032
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2G3
Immunogen
Purified recombinant fragment of human ARHGDIA (amino acids: FULL(1-204)) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17032 targets ARHGDIA in indirect ELISA, FACS, ICC, IF and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.
The MA5-17032 immunogen is purified recombinant fragment of human ARHGDIA (amino acids: FULL(1-204)) expressed in E. Coli.
MA5-17032 detects ARHGDIA which has a predicted molecular weight of approximately 26kDa.
Target Information
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARHGDIA; arhgdia {ECO:0000312|RGD:1359547}; epididymis secretory sperm binding protein Li 47e; GDI-1; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; rho GDI 1 {ECO:0000250|UniProtKB:P19803}; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP dissociation inhibitor (GDI) alpha isoform ARHGDIA_1; Rho GDP dissociation inhibitor (GDI) alpha isoform ARHGDIA_2; Rho GDP-dissociation inhibitor 1; rho GDP-dissociation inhibitor 1 {ECO:0000250|UniProtKB:P19803}; Rho-GDI alpha; rho-GDI alpha {ECO:0000250|UniProtKB:P19803}; unnamed protein product
Gene Aliases: 5330430M07Rik; ARHGDIA; Bles01; C87222; Gdi-1; Gdi1; GDIA1; HEL-S-47e; NPHS8; Rho-GDI; RhoDGI; RHOGDI; RHOGDI-1
UniProt ID: (Human) P52565, (Rat) Q5XI73, (Mouse) Q99PT1
Entrez Gene ID: (Human) 396, (Rat) 360678, (Mouse) 192662
Rho protein signal transduction
regulation of protein localization
regulation of Rho protein signal transduction
negative regulation of apoptotic process
semaphorin-plexin signaling pathway
regulation of synaptic vesicle cycle
regulation of cell shape
negative regulation of cell migration
positive regulation of GTPase activity
regulation of catalytic activity
cellular response to mechanical stimulus
cellular response to organic cyclic compound
cellular response to redox state
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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