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Invitrogen
GLUT4 Monoclonal Antibody (3G10A3)
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FIGURE: 1 / 12
GLUT4 Antibody (MA5-17176) in ICC/IF
Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT4 Monoclonal Antibody (3G10A3) (Product # MA5-17176, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:... View More
Product Details
MA5-17176
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3G10A3
Immunogen
Purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17176 targets SLC2A4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.
The MA5-17176 immunogen is purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli.
MA5-17176 detects SLC2A4 which has a predicted molecular weight of approximately 54.8kDa.
Target Information
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glucose transporter glut4; Glucose transporter type 4, insulin-responsive; GLUT-4; GT2; insulin-responsive glucose transporter; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 (facilitated glucose transporter), member 4; Solute carrier family 2, facilitated glucose transporter member 4; unnamed protein product
Gene Aliases: Glut-4; GLUT4; SLC2A4
UniProt ID: (Human) P14672, (Mouse) P14142
Entrez Gene ID: (Human) 6517, (Mouse) 20528
carbohydrate metabolic process
learning or memory
short-term memory
long-term memory
amylopectin biosynthetic process
positive regulation of brain-derived neurotrophic factor receptor signaling pathway
cellular response to insulin stimulus
glucose homeostasis
glucose import in response to insulin stimulus
response to ethanol
glucose import
brown fat cell differentiation
transmembrane transport
white fat cell proliferation
dehydroascorbic acid transport
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to osmotic stress
regulation of synaptic vesicle budding from presynaptic endocytic zone membrane
transport across blood-brain barrier
glucose transmembrane transport
transport
carbohydrate transport
glucose transport
proton transport
hexose transmembrane transport
cation transmembrane transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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