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Invitrogen
RT1.Bu Monoclonal Antibody (OX-3), FITC
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Product Details
MA5-17416
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OX-3
Immunogen
Rat thymocyte membrane glycoprotein
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 4-5mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Anti-rat RT1.Bu monoclonal antibody recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. Recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Thus, this antibody is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
Target Information
RT1.Bu is a gene located within the major histocompatibility complex (MHC) region of the rat genome, specifically part of the RT1 complex. The RT1 complex in rats is analogous to the human HLA and mouse H2 MHC regions and encompasses a variety of class I and class II genes involved in antigen presentation. RT1.Bu is one such class I MHC gene, encoding a protein essential for the presentation of endogenous peptides to cytotoxic T cells. This interaction is vital for the immune system's ability to recognize and eliminate infected or malignantly transformed cells. The RT1.Bu gene is crucial for studying immune responses, particularly in transplantation immunology and autoimmune disease models, due to its role in histocompatibility and immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activated B cell RT1Bl alpha chain; MHC class II antigen RT1.B alpha chain; rano class II histocompatibility antigen, B alpha chain; rat class I Bu; RT1 class II antigen, Ba chain; RT1-B alpha; RT1-Bu
Gene Aliases: BA1
Entrez Gene ID: (Rat) 309621
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
response to interferon-gamma
negative regulation of T cell proliferation
positive regulation of T cell differentiation
antigen processing and presentation of peptide antigen
cellular response to glucocorticoid stimulus
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