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Invitrogen
NOX2 Monoclonal Antibody (54.1)
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FIGURE: 1 / 7
NOX2 Antibody (MA5-18052) in ICC/IF
Immunocytochemistry analysis of NOX2 in paraformaldehyde fixed MCF7 cells. Samples were incubated with NOX2 monoclonal antibody (Product # MA5-18052) using a dilution of 1:100 for 1hr. Followed by Alexa Fluor® 488 secondary antibody at a 1:1,000 dilution, showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
Product Details
MA5-18052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
54.1
Immunogen
Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for NOX2/gp91phox (aa 382-389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100).
Western blot results show a band at 65 kDa, which matches the predicted molecular weight. For IHC applications, perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining.
Target Information
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 beta polypeptide; Cytochrome b-245 heavy chain; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP911; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; p91PHOX; Superoxide-generating NADPH oxidase heavy chain subunit; unnamed protein product
Gene Aliases: AMCBX2; CGD; CGDX; CYBB; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX
UniProt ID: (Human) P04839
Entrez Gene ID: (Human) 1536
protein binding
electron carrier activity
NAD(P)H oxidase activity
superoxide-generating NADPH oxidase activity
oxidoreductase activity
heme binding
voltage-gated proton channel activity
metal ion binding
protein heterodimerization activity
flavin adenine dinucleotide binding
oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
NADPH binding
FAD binding
superoxide-generating NADPH oxidase activity
superoxide metabolic process
ion transport
defense response
inflammatory response
response to nutrient
response to xenobiotic stimulus
positive regulation of tumor necrosis factor production
ion transmembrane transport
superoxide anion generation
innate immune response
response to ethanol
respiratory burst
positive regulation of angiogenesis
hydrogen peroxide biosynthetic process
cellular response to cadmium ion
cellular response to ethanol
cellular response to hypoxia
hypoxia-inducible factor-1alpha signaling pathway
response to aldosterone
cellular response to L-glutamine
response to angiotensin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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