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Phosphotyrosine Monoclonal Antibody (P-Tyr-01), Alexa Fluor™ 647
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FIGURE: 1 / 1
Phosphotyrosine Antibody (MA5-18172) in Flow
Separation of phosphotyrosine positive cells (red-filled) from phosphotyrosine negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of EGF stimulated A431 cells stained using anti-phosphotyrosine (P-Tyr-01) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18172), concentration in sample 3 µg/mL.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-18172
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
P-Tyr-01
Immunogen
Phosphotyrosine conjugated to bovine serum albumin
Conjugate
Alexa Fluor™ 647
Alexa Fluor™ 647
Alexa Fluor™ 647
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects tyrosine phosphorylation in activated cells.
Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Phosphotyrosine; pTyr; pY
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