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          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          AKT1 Monoclonal Antibody (281046), APC

          View all (157) AKT1 antibodies

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          Cite AKT1 Monoclonal Antibody (281046), APC

          Additional Information:
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          • Antibody Testing Data (1)
          AKT1 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          AKT1 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          AKT1 Antibody (MA5-23646) in Flow

          Flow cytometry of AKT1 in Jurkat human acute T cell leukemia cell line. Samples were incubated in AKT1 monoclonal antibody (Product # MA5-23646) or isotype control antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          AKT1 Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          AKT1 Monoclonal Antibody (281046), APC

          Product Details

          MA5-23646

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          10 µL/10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          281046

          Immunogen

          E. coli-derived recombinant human Akt1 Ser2-Ala480
          3D Epitope / Immunogen

          Conjugate

          APC APC APC
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          651/660 nm View spectra spectra

          Form

          Liquid

          Purification

          Protein A/G

          Storage buffer

          Buffered saline solution with 0.5% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2608970

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT; AKT1 kinase; AKT1M; Akt1m protein; C-AKT; CAKT; murine thymoma viral (v-akt) oncogene homolog 1; PKB; PKB ALPHA; PKB beta; PKB gamma; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; PROTEIN KINASE B ALPHA; protein kinase B-alpha; PROTO-ONCOGENE C-AKT; RAC; RAC protein kinase alpha RAC-PK alpha; RAC-ALPHA; RAC-ALPHA SERINE/THREONINE-PROTEIN KINASE; RAC-PK-ALPHA; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1; V-AKT MURINE THYMOMA VIRAL ONCOGENE-LIKE PROTEIN 1

          View more View less

          Gene Aliases: Akt; AKT1; PKB; PKB/Akt; PKBalpha; RAC

          View more View less

          UniProt ID: (Mouse) P31750, (Rat) P47196

          View more View less

          Entrez Gene ID: (Mouse) 11651, (Rat) 24185

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein kinase binding nitric-oxide synthase regulator activity identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding non-receptor serine/threonine protein kinase
          Process(es)
          protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation carbohydrate metabolic process glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation protein phosphorylation negative regulation of protein kinase activity transport apoptotic process activation-induced cell death of T cells inflammatory response cytoskeleton organization signal transduction multicellular organism development germ cell development nervous system development aging insulin receptor signaling pathway apoptotic mitochondrial changes carbohydrate transport response to hormone negative regulation of autophagy negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide response to oxidative stress hyaluronan metabolic process response to growth hormone response to insulin-like growth factor stimulus
          It has to be done as per old AB suggested Products section.

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