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Invitrogen
Granzyme B Monoclonal Antibody (351927), PE
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FIGURE: 1 / 1
Granzyme B Antibody (MA5-23688) in Flow
Flow cytometry of Granzyme B in NK‚92 human natural killer lymphoma cell line. Samples were incubated in Granzyme B monoclonal antibody (Product # MA5-23688) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
Product Details
MA5-23688
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
351927
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B Gly19-Tyr247
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
Target Information
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternatively spliced; C11; Cathepsin G-like 1; CTLA-1; CTSGL1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; fragmentin 2; Fragmentin-2; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GranzymeB; HLP; Human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; OTTHUMP00000028189; proteolytic serine esterase; SECT; serine protease; serine protease-like protein precursor; T-cell serine protease 1-3E
Gene Aliases: C11; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; GRB; GZMB; HLP; SECT
UniProt ID: (Human) P10144
Entrez Gene ID: (Human) 3002
plasma membrane repair
positive regulation of immune response to tumor cell
proteolysis
apoptotic process
protein secretion
negative regulation of translation
killing of cells of other organism
natural killer cell mediated cytotoxicity
ceramide biosynthetic process
proteolysis involved in cellular protein catabolic process
protein maturation
pyroptosis
granzyme-mediated programmed cell death signaling pathway
pyroptotic cell death
positive regulation of establishment of protein localization to mitochondrion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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