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Invitrogen
Nitrotryptophan Monoclonal Antibody (2D12)
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FIGURE: 1 / 3
Nitrotryptophan Antibody (MA5-27569) in ICC/IF
Immunofluorescent analysis of Nitrotryptophan in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Nitrotryptophan monoclonal antibody (Product # MA5-27569) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 min... View More
Product Details
MA5-27569
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2D12
Immunogen
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
Product Specific Information
A 1:1,000 dilution of MA5-27569 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
Target Information
6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO- modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress. Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2-). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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