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Invitrogen

Dicer Monoclonal Antibody (N167/7)

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Dicer Monoclonal Antibody (N167/7)

Product Details

MA5-27595

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N167/7

Immunogen

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735166

Product Specific Information

1 µg/mL of MA5-27595 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 215kDa.

This antibody was formerly sold as clone S167-7.

Target Information

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: antiviral Dicer; antiviral isoform of Dicer; DCR; DCR-1; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; double-strand-specific ribonuclease mDCR-1; helicase MOI; helicase with RNAse motif; helicase-moi; mDCR-1; unnamed protein product; variant of Dicer1

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Gene Aliases: 1110006F08Rik; aviD; D12Ertd7e; DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; mKIAA0928; MNG1; RMSE2

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UniProt ID: (Human) Q9UPY3

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Entrez Gene ID: (Human) 23405, (Rat) 299284, (Mouse) 192119

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Function(s)
nucleic acid binding RNA binding double-stranded RNA binding endoribonuclease activity ribonuclease III activity deoxyribonuclease I activity protein binding ATP binding endoribonuclease activity, producing 5'-phosphomonoesters protein domain specific binding siRNA binding regulatory RNA binding pre-miRNA binding DNA binding hydrolase activity miRNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter meiotic spindle organization angiogenesis trophectodermal cell proliferation hair follicle development apoptotic DNA fragmentation RNA processing spermatogonial cell division cell proliferation post-embryonic development zygote asymmetric cell division regulation of gene expression positive regulation of endothelial cell migration negative regulation of Schwann cell proliferation positive regulation of gene expression negative regulation of gene expression regulation of muscle cell apoptotic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of Schwann cell differentiation myoblast differentiation involved in skeletal muscle regeneration posttranscriptional gene silencing stem cell population maintenance spinal cord motor neuron differentiation nerve development olfactory bulb interneuron differentiation cerebral cortex development lung development embryonic limb morphogenesis production of siRNA involved in RNA interference pre-miRNA processing hair follicle morphogenesis pericentric heterochromatin assembly regulation of myelination positive regulation of myelination peripheral nervous system myelin formation negative regulation of tumor necrosis factor production positive regulation of collagen biosynthetic process embryonic hindlimb morphogenesis posttranscriptional gene silencing by RNA production of miRNAs involved in gene silencing by miRNA multicellular organism growth regulation of odontogenesis of dentin-containing tooth regulation of viral genome replication regulation of regulatory T cell differentiation regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter mRNA stabilization spleen development digestive tract development reproductive structure development regulation of oligodendrocyte differentiation epidermis morphogenesis branching morphogenesis of an epithelial tube neuron projection morphogenesis regulation of inflammatory response regulation of neurogenesis cartilage development spindle assembly regulation of multicellular organismal process regulation of RNA metabolic process defense response to virus regulation of cell cycle cardiac muscle cell development inner ear receptor cell development negative regulation of glial cell proliferation intestinal epithelial cell development cardiac neural crest cell development involved in outflow tract morphogenesis ganglion development regulation of enamel mineralization cellular response to hydrogen peroxide small RNA loading onto RISC cellular response to glucose stimulus hair follicle cell proliferation cellular response to tumor necrosis factor cellular response to hypoxia positive regulation of establishment of endothelial barrier positive regulation of hepatic stellate cell proliferation positive regulation of endothelial cell-matrix adhesion via fibronectin positive regulation of vascular endothelial cell proliferation regulation of miRNA metabolic process positive regulation of miRNA metabolic process regulation of stem cell differentiation rRNA processing regulation of Notch signaling pathway global gene silencing by mRNA cleavage miRNA metabolic process tRNA catabolic process
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