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Invitrogen
NRCAM Monoclonal Antibody (N364/51)
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FIGURE: 1 / 2
NRCAM Antibody (MA5-27673) in ICC/IF
Immunofluorescent analysis of NRCAM in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NRCAM monoclonal antibody (Product # MA5-27673) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) NrCAM Antibody, D) Merged image. Magnification... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27673
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N364/51
Immunogen
Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27673 was sufficient for detection of NrCAM in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 160kDa.
This antibody was formerly sold as clone S364-51.
Target Information
Neuron-glia-related cell adhesion molecule (NRCAM) is a gene located on chromosome 7q31.1, encoding a protein that belongs to the immunoglobulin superfamily of cell adhesion molecules. NRCAM is expressed in the nervous system and plays a vital role in the development and maintenance of neural connections. This adhesion molecule facilitates neuron-neuron and neuron-glia interactions, impacting processes such as axon guidance, synapse formation, and cell signaling, which are crucial for neural development and plasticity. NRCAM is involved in the modulation of signal transduction pathways that influence neuronal growth and repair, contributing to the stabilization of synaptic structures. Alterations in NRCAM expression or function have been linked to neurodevelopmental disorders, including autism spectrum disorders, and certain psychiatric conditions, reflecting its importance in maintaining neural circuit integrity. Research into NRCAM continues to explore its therapeutic potential in neurobiology and its implications in neurological and psychiatric disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ankyrin-binding cell adhesion molecule NrCAM; KIAA0343; mBravo; MGC138845; MGC138846; neuron-glia-CAM-related cell adhesion molecule; neuronal surface protein Bravo; ng-CAM-related; NgCAM-related cell adhesion molecule; Nr CAM; nr-CAM; NrCAM; rBravo; unnamed protein product
Gene Aliases: Bravo; C030017F07Rik; C130076O07Rik; mKIAA0343; NEDNMS
UniProt ID: (Human) Q92823, (Mouse) Q810U4
Entrez Gene ID: (Human) 4897, (Rat) 497815, (Mouse) 319504
angiogenesis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
axon guidance
central nervous system development
protein localization
regulation of neuron projection development
neuronal action potential propagation
retinal ganglion cell axon guidance
heterotypic cell-cell adhesion
clustering of voltage-gated sodium channels
dendrite self-avoidance
cell-cell adhesion
regulation of postsynapse organization
neuron migration
axonogenesis
axonal fasciculation
synapse assembly
brain development
regulation of axon extension
positive regulation of neuron differentiation
synapse organization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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