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Invitrogen
ERp57 Monoclonal Antibody (4F9)
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FIGURE: 1 / 2
ERp57 Antibody (MA5-27689) in ICC/IF
Immunofluorescent analysis of ERp57 in human HeLa cells (human cervical cancer). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ERp57 monoclonal antibody (Product # MA5-27689) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, (B) Phalloidin Texas Red F-Actin stain, (C) ERp57 Antibody, (D) Merged image. Magnifica... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27689
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4F9
Immunogen
Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of MA5-27689 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Detects approximately 57 kDa protein.
Target Information
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER p57; ER protein 57; ER protein 60; ERp 57; glucose regulated protein, 58kDa; GRP 57; GRP 58; PDIA 3; phospholipase C-alpha; PI PLC; protein disulfide isomerase-associated 3; unnamed protein product
Gene Aliases: ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC
UniProt ID: (Human) P30101
Entrez Gene ID: (Human) 2923
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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