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Invitrogen
GABRA5 Monoclonal Antibody (N415/24)
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FIGURE: 1 / 2
GABRA5 Antibody (MA5-27700) in ICC/IF
Immunofluorescent analysis of GABRA5 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GABRA5 monoclonal antibody (Product # MA5-27700) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GABA-A Receptor Alpha5 Antibody, D) Merged i... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N415/24
Immunogen
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-27700 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
This antibody was formerly sold as clone S415-24.
Target Information
GABRA5 is a part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor; GABA(A) receptor subunit alpha-5; GABA-A receptor alpha-5 subunit; GABAAR subunit alpha-5; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 5; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid type A receptor alpha 5 subunit; gamma-aminobutyric acid type A receptor alpha5 subunit; ligand-gated ion channel subunit; unnamed protein product
Gene Aliases: A230018I05Rik; DEE79; EIEE79
UniProt ID: (Human) P31644, (Rat) P19969, (Mouse) Q8BHJ7
Entrez Gene ID: (Human) 2558, (Rat) 29707, (Mouse) 110886
transmembrane signaling receptor activity
GABA-A receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
GABA-gated chloride ion channel activity
signaling receptor activity
GABA receptor binding
ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
chloride channel activity
protein binding
behavioral fear response
ion transport
chloride transport
gamma-aminobutyric acid signaling pathway
chemical synaptic transmission
associative learning
ion transmembrane transport
auditory receptor cell differentiation
neuron development
synaptic transmission, GABAergic
regulation of postsynaptic membrane potential
inner ear receptor cell development
innervation
cochlea development
regulation of presynaptic membrane potential
chloride transmembrane transport
inhibitory synapse assembly
signal transduction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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