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Invitrogen
PD-L1 (CD274) Recombinant Rabbit Monoclonal Antibody (RM320)
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FIGURE: 1 / 12
PD-L1 (CD274) Antibody (MA5-27896) in ICC/IF
Immunofluorescent analysis of PD-L1 was performed using PD-L1 (CD274) Recombinant Rabbit Monoclonal Antibody (RM320) (Product # MA5-27896, 1:400) on 70-80% confluent untreated THP-1 cells and THP-1 cells treated with PMA (100 nM for 24 hours) and further polarized into M1 macrophages using LPS (20 pg/mL) and human interferon gamma (20 ng/mL) for 48 hours. The cells were fixed with 4% paraformaldehyde for 10... View More
Product Details
MA5-27896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM320
Immunogen
A peptide corresponding to the C-terminus of human PD-L1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B7 homolog 1; B7-H1; B7-H1,PD-L1; CD274; CD274 antigen; costimulatory molecule; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death-1 ligand 1; sCD274; similar to Homo sapiens B7-1 and B7-2; soluble CD274; soluble PD L1; soluble PD-L1; B7-H1; sPD L1; unnamed protein product
Gene Aliases: B7-H; B7H1; CD274; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
UniProt ID: (Human) Q9NZQ7
Entrez Gene ID: (Human) 29126
adaptive immune response
immune system process
negative regulation of T cell mediated immune response to tumor cell
immune response
signal transduction
cell surface receptor signaling pathway
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
positive regulation of interleukin-10 production
response to cytokine
TRIF-dependent toll-like receptor signaling pathway
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of transcription, DNA-templated
regulation of activated T cell proliferation
negative regulation of activated T cell proliferation
negative regulation of T cell activation
regulation of T cell apoptotic process
cellular response to lipopolysaccharide
negative regulation of tumor necrosis factor superfamily cytokine production
regulation of activated CD4-positive, alpha-beta T cell apoptotic process
positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process
negative regulation of CD4-positive, alpha-beta T cell proliferation
negative regulation of CD8-positive, alpha-beta T cell activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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