Flow cytometry of CD27 in red cell lysed porcine blood. Sample was incubated with CD27 monoclonal antibody (Product # MA5-28339) and gated on lymphocytes. Figures are arranged as follows: A) RPE conjugated mouse anti porcine CD3 and APC conjugated mouse IgG1 isotype control. B) RPE conjugated mouse anti porcine CD3 and APC conjugated mouse anti porcine CD27.

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Product Details

MA5-28339

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

Product Specifications

Species Reactivity

Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

B30C7

Immunogen

Porcine peripheral blood monocytes

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Protein A

Storage buffer

PBS with 5% sucrose, 1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2745302

Product Specific Information

Mouse anti Pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family.

Reconstitute with 1 mL distilled water.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: LPFS2; sCD27; soluble CD27; TNFSF7

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Entrez Gene ID: (Pig) 100520023