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Invitrogen
CD45 Monoclonal Antibody (K252.1E4), FITC
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FIGURE: 1 / 1
CD45 Antibody (MA5-28383) in Flow
Flow cytometry of CD45 in red cell lysed porcine blood. Sample was incubated with CD45 monoclonal antibody (Product # MA5-28383) and gated on lymphocytes. Figures are arranged as follows: A) FITC conjugated mouse anti porcine CD3 and Alexa Fluor 647 conjugated mouse IgG1 isotype control. B) FITC conjugated mouse anti porcine CD3 and Alexa Fluor 647 conjugated mouse anti porcine CD45.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-28383
Product Specifications
Species Reactivity
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K252.1E4
Immunogen
Porcine Peripheral Blood lymphocytes
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mouse anti Pig CD45, clone K252.1E4 recognizes an epitope common to all porcine CD45 isoforms (Schnitzlein et al. 1998).
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD45 antigen; protein tyrosine phosphatase; alternatively spliced; receptor-type tyrosine-protein phosphatase C; T220 and B220
Gene Aliases: CD45
Entrez Gene ID: (Pig) 100522631
Disclaimer
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