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Invitrogen
Phospho-CD247 (CD3 zeta) (Tyr72) Monoclonal Antibody (EM-26)
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FIGURE: 1 / 5
Phospho-CD247 (CD3 zeta) (Tyr72) Antibody (MA5-28537) in ICC/IF
Immunocytochemistry detection of phosphorylated CD3 zeta (EM-26; light blue) Monoclonal antibody (Product # MA5-28537) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.
Product Details
MA5-28537
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
EM-26
Immunogen
A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Western Blot: non-reducing conditions recommended.
Target Information
CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD247; CD247 antigen; CD247 antigen, zeta subunit; CD3 antigen, zeta polypeptide; CD3-epsilon; CD3-zeta/eta; CD3e antigen; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 eta chain; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; T3E; TCR zeta chain; TCRE
Gene Aliases: 4930549J05Rik; A430104F18Rik; AW552088; CD247; Cd3; Cd3-eta; CD3-ZETA; CD3H; CD3Q; CD3Z; Cd3zeta; IMD25; T3Z; Tcrk; TCRZ
UniProt ID: (Human) P20963, (Mouse) P24161
Entrez Gene ID: (Human) 919, (Mouse) 12503
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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