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Invitrogen
CD107b (LAMP-2) Monoclonal Antibody (H4B4)
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FIGURE: 1 / 4
CD107b (LAMP-2) Antibody (MA5-28577) in WB
Western Blot analysis of CD107b (LAMP-2) using CD107b (LAMP-2) Monoclonal Antibody (H4B4) (Product # MA5-28577). Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of SK-MEL30, MCF-7, THP-1, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody (1 µg/mL), followed by Goat-anti-Mouse IgG (green). Mouse anti-GAPDH m... View More
Product Details
MA5-28577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
H4B4
Immunogen
Human PBMC
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular/luminal epitope of CD107b / LAMP-2, an extensively glycosylated 100-120 kDa widely expressed lysosome-associated protein.
Target Information
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LAMP-2C; LGP-96; Lysosome-associated membrane glycoprotein 2; RP23-193O17.2; unnamed protein product
Gene Aliases: CD107b; DND; LAMP-2; LAMP2; LAMPB; LGP-96; LGP110
UniProt ID: (Human) P13473
Entrez Gene ID: (Human) 3920
protein targeting
autophagy
lysosomal lumen acidification
cellular response to starvation
protein import
protein catabolic process
negative regulation of protein complex assembly
regulation of protein stability
lysosomal lumen pH elevation
muscle cell cellular homeostasis
protein stabilization
chaperone-mediated autophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
autophagosome maturation
negative regulation of NLRP3 inflammasome complex assembly
hydrogen ion transmembrane transport
lysosomal protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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