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          • Primary Antibodies ›
          • IKAROS Antibodies

          Invitrogen

          IKAROS Monoclonal Antibody (4E9), PE

          View all (21) IKAROS antibodies

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          Cite IKAROS Monoclonal Antibody (4E9), PE

          Additional Information:
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          • Antibody Testing Data (2)
          IKAROS Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          IKAROS Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          IKAROS Antibody (MA5-28612) in Flow

          Flow Cytometry analysis of IKAROS using IKAROS Monoclonal Antibody (4E9), PE (Product # MA5-28612). Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Ikaros (4E9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          IKAROS Antibody in Flow Cytometry (Flow)
          IKAROS Antibody in Flow Cytometry (Flow)
          IKAROS Monoclonal Antibody (4E9), PE

          Product Details

          MA5-28612

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          10 µL/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4E9

          Immunogen

          Recombinant human Ikaros (C-terminal region)
          3D Epitope / Immunogen

          Conjugate

          PE PE PE
          • Unconjugated
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Purification

          Size-exclusion chromatography

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          15mM sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2745571

          Product Specific Information

          This antibody recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.

          Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension.

          Target Information

          DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: CLL-associated antigen KW-6; DNA-binding protein Ikaros; hIk-1; Ikaros family zinc finger protein 1; Ikaros zinc finger protein; zinc finger protein subfamily 1A 1; DNA-binding protein Ikaros; CLL-associated antigen KW-6; Ikaros family zinc finger protein 1; lymphoid transcription factor LyF-1; lymphocyte-restricted zinc finger DNA binding protein; Lymphoid transcription factor LyF-1; predicted protein of HQ0758; protein phosphatase 1, regulatory subunit 92; unnamed protein product; zinc finger protein, subfamily 1A, 1 (Ikaros); zinc finger transcription factor

          View more View less

          Gene Aliases: 5832432G11Rik; CVID13; hlk-1; Hs.54452; IK1; IKAROS; IKZF1; LyF-1; LYF1; mKIAA4227; PPP1R92; PRO0758; Zfpn1a1; ZNFN1A1

          View more View less

          UniProt ID: (Human) Q13422

          View more View less

          Entrez Gene ID: (Human) 10320, (Mouse) 22778

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding protein domain specific binding identical protein binding metal ion binding C2H2 zinc finger transcription factor
          Process(es)
          chromatin organization regulation of transcription from RNA polymerase II promoter mesoderm development lymphocyte differentiation erythrocyte differentiation negative regulation of transcription, DNA-templated
          It has to be done as per old AB suggested Products section.

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