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          • Primary Antibodies ›
          • Human Serum Albumin Antibodies

          Invitrogen

          Human Serum Albumin Recombinant Rabbit Monoclonal Antibody (101)

          Advanced Verification
          2 Published Figures
          2 References
          View all (47) Human Serum Albumin antibodies

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          Cite Human Serum Albumin Recombinant Rabbit Monoclonal Antibody (101)

          Additional Information:
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          • Antibody Testing Data (5)
          • Published Figures (2)
          • Advanced Verification (1)
          Human Serum Albumin Antibody in Immunocytochemistry (ICC/IF)
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          Human Serum Albumin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Human Serum Albumin Antibody (MA5-29022) in ICC/IF

          Immunofluorescence staining of Human HSA in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Human Serum Albumin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29022, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Human Serum Albumin Antibody in Immunocytochemistry (ICC/IF)
          Human Serum Albumin Antibody in Western Blot (WB)
          Human Serum Albumin Antibody in Western Blot (WB)
          Human Serum Albumin Antibody in Flow Cytometry (Flow)
          Human Serum Albumin Antibody in Immunoprecipitation (IP)
          Human Serum Albumin Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Human Serum Albumin Antibody in Flow Cytometry (Flow)
          Human Serum Albumin Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Human Serum Albumin Recombinant Rabbit Monoclonal Antibody (101)

          Product Details

          MA5-29022

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:20-1:100
          -

          Flow Cytometry (Flow)

          1:25-1:100
          View 1 publication 1 publication

          ELISA (ELISA)

          1:5,000-1:10,000
          -

          Immunoprecipitation (IP)

          0.5-2 µL/mg of lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          101

          Immunogen

          Human Serum Albumin/HSA
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • FITC
          • HRP
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 6

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2784959

          Product Specific Information

          This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          This antibody has specificity for Human Serum Albumin/HSA.

          Target Information

          Albumin is a soluble, monomeric protein with a molecular weight of approximately 65-67 kDa that comprises about one-half of the blood serum protein. Human serum albumin (HAS) is the most abundant protein in human blood plasma, and has a serum half-life of approximately 20 days. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular, unglycosylated serum protein synthesized in the liver as pre-pro-albumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough `produce the secreted albumin. Serum albumin has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. The main function of albumin is the regulation of the colloidal osmotic pressure of blood.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DKFZp779N1935; GIG20; GIG42; serum albumin; unnamed protein product

          View more View less

          Gene Aliases: FDAHT; HSA; PRO0883; PRO0903; PRO1341

          View more View less

          UniProt ID: (Human) P02768

          View more View less

          Entrez Gene ID: (Human) 213

          View more View less

          Function(s)
          DNA binding fatty acid binding copper ion binding protein binding toxic substance binding antioxidant activity oxygen binding heme binding pyridoxal phosphate binding identical protein binding chaperone binding molecular carrier activity exogenous protein binding enterobactin binding
          Process(es)
          heme biosynthetic process cellular response to starvation bilirubin transport response to nutrient levels cellular response to oxidative stress heme catabolic process negative regulation of mitochondrial depolarization cellular response to calcium ion starvation cellular oxidant detoxification
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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