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Invitrogen
CAR Recombinant Rabbit Monoclonal Antibody (271)
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FIGURE: 1 / 2
CAR Antibody (MA5-29208) in ICC/IF
Immunofluorescence staining of Human CAR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CAR Recombinant Rabbit Monoclonal Antibody (271) (Product # MA5-29208, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Product Details
MA5-29208
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
271
Immunogen
Recombinant Human CXADR/CAR protein (Met1-Gly237)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7, with 10% trehalose
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CXADR/CAR.
Target Information
The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 46 kD coxsackievirus and adenovirus receptor (CAR) protein; alternatively spliced; CAR; coxsackie virus and adenovirus receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3-binding protein; CXADR Ig-like cell adhesion molecule; HCVADR; membranous splice variant of CAR1; unnamed protein product
Gene Aliases: CAR; CAR4/6; CXADR; HCAR
UniProt ID: (Human) P78310
Entrez Gene ID: (Human) 1525
mitochondrion organization
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
heart development
germ cell migration
epithelial structure maintenance
actin cytoskeleton organization
neutrophil chemotaxis
homotypic cell-cell adhesion
cell-cell junction organization
gamma-delta T cell activation
viral entry into host cell
defense response to virus
cardiac muscle cell development
transepithelial transport
AV node cell to bundle of His cell communication
AV node cell-bundle of His cell adhesion involved in cell communication
regulation of AV node cell action potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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