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Invitrogen
Phospho-Stat3 (Tyr705) Recombinant Rabbit Monoclonal Antibody (Stat3Y705-B12)
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FIGURE: 1 / 4
Phospho-Stat3 (Tyr705) Antibody (MA5-36899) in Flow
Flow cytometry of Phospho-Stat3 (Tyr705) using Jurkat cells. Samples were incubated in monoclonal Phospho-Stat3 (Tyr705) antibody (Product # MA5-36899) with a dilution of 5 ng/mL. Images correspond with the following conditions: negative control (blue) untreated (red) treated with IFNa IL-4 and pervanadate (green) treated/blocked with phospho-peptide (brown).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-36899
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Stat3Y705-B12
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr705 of human phospho Stat3.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol, 0.10% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acute phase response factor; Acute-phase response factor; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; STAT
Gene Aliases: 1110034C02Rik; APRF; AW109958; STAT3
UniProt ID: (Mouse) P42227
Entrez Gene ID: (Mouse) 20848
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
RNA polymerase II repressing transcription factor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
signal transducer activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
transcription factor binding
protein kinase binding
protein phosphatase binding
CCR5 chemokine receptor binding
glucocorticoid receptor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein dimerization activity
DNA-binding transcription factor
temperature homeostasis
eye photoreceptor cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein import into nucleus
acute-phase response
signal transduction
JAK-STAT cascade
cell proliferation
negative regulation of cell proliferation
negative regulation of hydrogen peroxide biosynthetic process
phosphorylation
cytokine-mediated signaling pathway
stem cell population maintenance
sexual reproduction
intracellular receptor signaling pathway
response to estradiol
cellular response to hormone stimulus
response to cytokine
regulation of multicellular organism growth
glucose homeostasis
eating behavior
negative regulation of apoptotic process
positive regulation of Notch signaling pathway
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of mitochondrial membrane permeability
astrocyte differentiation
radial glial cell differentiation
growth hormone receptor signaling pathway
JAK-STAT cascade involved in growth hormone signaling pathway
negative regulation of cell death
interleukin-6-mediated signaling pathway
negative regulation of neuron death
positive regulation of growth factor dependent skeletal muscle satellite cell proliferation
positive regulation of ATP biosynthetic process
negative regulation of neuron migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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