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          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          Phospho-Akt1 (Ser473) Recombinant Rabbit Monoclonal Antibody (AktS473-C7), PE

          View all (175) AKT1 antibodies

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          Cite Phospho-Akt1 (Ser473) Recombinant Rabbit Monoclonal Antibody (AktS473-C7), PE

          Additional Information:
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          • Antibody Testing Data (1)
          Phospho-Akt1 (Ser473) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-Akt1 (Ser473) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-Akt1 (Ser473) Antibody (MA5-36904) in Flow

          Flow cytometry of Phospho-Akt1 (Ser473) using Jurkat cells. Samples were incubated in monoclonal Phospho-Akt1 (Ser473) antibody (Product # MA5-36904). Images correspond with the following conditions: treated with LY294002 and unstained (blue) as negative control or treated with LY294002 (red), with FBS (green). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          Phospho-Akt1 (Ser473) Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-Akt1 (Ser473) Recombinant Rabbit Monoclonal Antibody (AktS473-C7), PE

          Product Details

          MA5-36904

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG, kappa

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          AktS473-C7

          Immunogen

          A synthetic phospho-peptide corresponding to residues surrounding Ser473 of human phospho Akt1.
          3D Epitope / Immunogen

          Conjugate

          PE PE PE
          • Unconjugated
          • APC 
          • FITC
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Purification

          Protein A/G

          Storage buffer

          PBS with 0.20% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2896839

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT; AKT1 kinase; AKT1M; Akt1m protein; C-AKT; CAKT; PKB ALPHA; PKB beta; PKB gamma; PKB-ALPHA; PKBG; PRKBA; PROTEIN KINASE B ALPHA; protein kinase B-alpha; PROTO-ONCOGENE C-AKT; proto-oncogene product; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-ALPHA SERINE/THREONINE-PROTEIN KINASE; RAC-PK-ALPHA; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; thymoma viral proto-oncogene 1; tumor rejection antigen peptide pRLib; unnamed protein product; V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1; V-AKT MURINE THYMOMA VIRAL ONCOGENE-LIKE PROTEIN 1

          View more View less

          Gene Aliases: AKT; LTR-akt; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

          View more View less

          UniProt ID: (Human) P31749, (Mouse) P31750

          View more View less

          Entrez Gene ID: (Human) 207, (Mouse) 11651

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding protein kinase C binding GTPase activating protein binding protein phosphatase 2A binding protein serine/threonine kinase binding
          Process(es)
          osteoblast differentiation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway regulation of glycogen biosynthetic process protein phosphorylation nitric oxide biosynthetic process activation-induced cell death of T cells response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway cell proliferation insulin receptor signaling pathway response to heat response to hormone negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of cell growth regulation of cell migration positive regulation of cell migration T cell costimulation negative regulation of protein ubiquitination TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to stress response to fluid shear stress intracellular signal transduction interleukin-18-mediated signaling pathway positive regulation of protein import into nucleus regulation of apoptotic process negative regulation of apoptotic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of glucose import positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation positive regulation of protein metabolic process positive regulation of protein kinase B signaling excitatory postsynaptic potential response to growth hormone mammary gland epithelial cell differentiation response to UV-A protein localization to mitochondrion response to growth factor cellular response to epidermal growth factor stimulus maintenance of protein location in mitochondrion cellular response to rapamycin negative regulation of release of cytochrome c from mitochondria vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway maternal placenta development cytoplasmic translation glycogen metabolic process glycogen biosynthetic process glucose metabolic process gluconeogenesis translation translational initiation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport inflammatory response cellular response to DNA damage stimulus cytoskeleton organization smoothened signaling pathway germ cell development apoptotic mitochondrial changes cellular response to starvation gene expression positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of mitochondrial membrane potential response to muscle activity phosphorylation cell migration protein ubiquitination spinal cord development lateral motor column neuron differentiation oogenesis stage protein catabolic process negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels cellular response to nutrient levels response to food peripheral nervous system myelin maintenance regulation of protein localization negative regulation of superoxide anion generation negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation cellular response to heat protein K29-linked ubiquitination cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus cellular response to decreased oxygen levels SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling glucose homeostasis Golgi to plasma membrane protein transport positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cholesterol biosynthetic process positive regulation of cilium assembly negative regulation of cell size positive regulation of vasoconstriction negative regulation of translational initiation positive regulation of translational initiation negative regulation of JNK cascade positive regulation of organ growth protein autophosphorylation protein stabilization striated muscle cell differentiation protein maturation regulation of vesicle-mediated transport glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development protein localization to cilium protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to mechanical stimulus cellular response to calcium ion cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia cellular hyperosmotic salinity response tricarboxylic acid metabolic process cellular response to sorbitol protein K6-linked ubiquitination cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis tRNA (guanine-N7)-methylation negative regulation of calcium import into the mitochondrion cGAS/STING signaling pathway cellular response to peptide regulation of aerobic respiration negative regulation of TORC1 signaling
          It has to be done as per old AB suggested Products section.

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