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Phospho-Btk (Tyr223) Recombinant Rabbit Monoclonal Antibody (BtkY223-B4), FITC

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Phospho-Btk (Tyr223) Recombinant Rabbit Monoclonal Antibody (BtkY223-B4), FITC

Product Details

MA5-36925

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

BtkY223-B4

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk.
3D Epitope / Immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896860

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily of tyrosine kinases. Mutations in the BTK gene have been linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; frameshift mutation results in truncated protein; Kinase EMB; MGC126261; MGC126262; OTTHUMP00000023677; RP1-164F3.2; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); unnamed protein product; X-linked immune deficiency

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Gene Aliases: AGMX1; AI528679; AT; ATK; BPK; BTK; IGHD3; IMD1; PSCTK1; xid; XLA

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UniProt ID: (Human) Q06187, (Mouse) P35991

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Entrez Gene ID: (Human) 695, (Mouse) 12229

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Function(s)
nucleotide binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding zinc ion binding lipid binding phospholipase activator activity kinase activity transferase activity identical protein binding phospholipase binding metal ion binding transferase activity, transferring phosphorus-containing groups
Process(es)
neutrophil homeostasis positive regulation of type III hypersensitivity positive regulation of type I hypersensitivity negative regulation of cytokine production adaptive immune response B cell affinity maturation immune system process histamine secretion by mast cell positive regulation of immunoglobulin production regulation of B cell cytokine production MyD88-dependent toll-like receptor signaling pathway regulation of B cell apoptotic process apoptotic process mesoderm development peptidyl-tyrosine phosphorylation calcium-mediated signaling proteoglycan catabolic process negative regulation of B cell proliferation positive regulation of B cell proliferation positive regulation of type I interferon production response to lipopolysaccharide negative regulation of interleukin-10 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production cellular response to reactive oxygen species intracellular signal transduction Fc-epsilon receptor signaling pathway B cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of B cell differentiation cell maturation positive regulation of phagocytosis T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell activation negative regulation of B cell activation positive regulation of NF-kappaB transcription factor activity monocyte proliferation negative regulation of leukocyte proliferation cellular response to molecule of fungal origin apoptotic signaling pathway cellular response to interleukin-7 cGAS/STING signaling pathway positive regulation of cGAS/STING signaling pathway positive regulation of interleukin-17A production positive regulation of NLRP3 inflammasome complex assembly positive regulation of synoviocyte proliferation eosinophil homeostasis transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway I-kappaB kinase/NF-kappaB signaling response to organic substance phosphorylation cell differentiation peptidyl-tyrosine autophosphorylation regulation of cell proliferation protein autophosphorylation
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