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Invitrogen
Phospho-PLCg1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (PLCG1Y783-C4), PE
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FIGURE: 1 / 1
Phospho-PLCg1 (Tyr783) Antibody (MA5-37027) in Flow
Flow cytometry of Phospho-PLCg1 (Tyr783) using HeLa cells. Samples were incubated in monoclonal Phospho-PLCg1 (Tyr783) antibody (Product # MA5-37027). Images correspond with the following conditions: unstained imatinib treated (blue), stained treated with imatinib (red), with pervanadate (green).
Product Details
MA5-37027
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PLCG1Y783-C4
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr783 of human phospho PLCgamma1.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; phospholipase C-gamma; Phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C2; PLC gamma1; PLC-148; PLC-gamma-1; PLC-II; PLC2I; triphosphoinositide phosphodiesterase; unnamed protein product
Gene Aliases: AI894140; Cded; IDAA; NCKAP3; Plc-1; Plc-gamma1; PLC-II; PLC1; PLC148; Plcg-1; PLCG1; PLCgamma1
UniProt ID: (Human) P19174, (Mouse) Q62077
Entrez Gene ID: (Human) 5335, (Mouse) 18803
phosphatidylinositol phospholipase C activity
phospholipase C activity
guanyl-nucleotide exchange factor activity
neurotrophin TRKA receptor binding
calcium ion binding
protein binding
phosphoric diester hydrolase activity
hydrolase activity
protein kinase binding
metal ion binding
calcium-dependent phospholipase C activity
phosphatidylinositol phospholipase C activity
signal transducer activity
insulin receptor binding
receptor tyrosine kinase binding
glutamate receptor binding
phosphoprotein binding
phospholipase
negative regulation of inflammatory response to antigenic stimulus
lipid metabolic process
signal transduction
epidermal growth factor receptor signaling pathway
phospholipid catabolic process
positive regulation of epithelial cell migration
lipid catabolic process
cell migration
calcium-mediated signaling
intracellular signal transduction
Fc-epsilon receptor signaling pathway
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
phosphatidylinositol metabolic process
phosphatidylinositol-mediated signaling
antigen receptor-mediated signaling pathway
T cell receptor signaling pathway
release of sequestered calcium ion into cytosol
positive regulation of release of sequestered calcium ion into cytosol
regulation of sequestering of calcium ion
cellular response to epidermal growth factor stimulus
positive regulation of vascular endothelial cell proliferation
positive regulation of endothelial cell apoptotic process
in utero embryonic development
calcium ion transport
protein secretion
inositol trisphosphate biosynthetic process
regulation of store-operated calcium channel activity
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