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Invitrogen
Phospho-SHP-1 (Tyr536) Recombinant Rabbit Monoclonal Antibody (Shp1Y536-2A7)
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FIGURE: 1 / 1
Phospho-SHP-1 (Tyr536) Antibody (MA5-37128) in Flow
Flow cytometry of Phospho-Shp1 (Tyr536) using Ramos cells. Samples were incubated in monoclonal Phospho-Shp1 (Tyr536) antibody (Product # MA5-37128) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with pervanadate (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-37128
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Shp1Y536-2A7
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr536 of human phospho Shp1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol, 0.10% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 3.1.3.48; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP-1; PTN6; unnamed protein product
Gene Aliases: HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; SHP1
UniProt ID: (Human) P29350
Entrez Gene ID: (Human) 5777
phosphotyrosine binding
protein tyrosine phosphatase activity
non-membrane spanning protein tyrosine phosphatase activity
transmembrane receptor protein tyrosine phosphatase activity
protein binding
SH3 domain binding
protein kinase binding
SH2 domain binding
cell adhesion molecule binding
phosphorylation-dependent protein binding
mitotic cell cycle
protein dephosphorylation
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of angiogenesis
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
T cell costimulation
negative regulation of lipopolysaccharide-mediated signaling pathway
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
negative regulation of mast cell activation involved in immune response
peptidyl-tyrosine dephosphorylation
T cell activation
negative regulation of innate immune response
negative regulation of inflammatory response
negative regulation of antigen receptor-mediated signaling pathway
negative regulation of B cell receptor signaling pathway
positive regulation of protein kinase B signaling
regulation of type I interferon-mediated signaling pathway
regulation of ERK1 and ERK2 cascade
negative regulation of inflammatory response to wounding
CD27 signaling pathway
negative regulation of neutrophil activation
epididymis development
regulation of G1/S transition of mitotic cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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