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Phospho-Aurora A/B/C (Thr288, Thr232, Thr198) Recombinant Rabbit Monoclonal Antibody (AuroraABC-CC12), FITC

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Phospho-Aurora A/B/C (Thr288, Thr232, Thr198) Recombinant Rabbit Monoclonal Antibody (AuroraABC-CC12), FITC

Product Details

MA5-37154

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL/1x10^6 cells
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

AuroraABC-CC12

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198).
3D Epitope / Immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.20% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897088

Target Information

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AIM-1; ARK-1; ARK-2; ARK-3; Aurora 1; Aurora 2; Aurora 3; Aurora kinase A; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora kinase C; Aurora- and IPL1-like midbody-associated protein 1; aurora- and Ipl1-like midbody-associated protein 1 homolog; aurora-1; aurora-B; aurora-related kinase 2; aurora-related kinase 3; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora/IPL1-related kinase 2; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 2; Breast tumor-amplified kinase; epididymis secretory protein Li 90; Ipl1- and aurora-related kinase 1; protein kinase; chromosomal passenger protein; protein phosphatase 1, regulatory subunit 47; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine protein kinase 15; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase aurora-B; Serine/threonine-protein kinase aurora-C; Serine/threonine-protein kinase Ayk1; STK-1; unnamed protein product

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Gene Aliases: AIE2; AIK; AIK2; AIK3; AIM-1; AIM1; AIRK1; AIRK2; AIRK3; ARK-2; ARK1; ARK2; ARK3; AURA; AurB; AurC; AURKA; AURKB; aurkb-sv1; aurkb-sv2; AURKC; aurora-C; AYK1; BTAK; HEL-S-90; IAK1; IPL1; PPP1R47; PPP1R48; SPGF5; STK-1; STK1; STK12; STK13; STK15; STK5; STK6; STK7

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UniProt ID: (Human) O14965, (Human) Q96GD4, (Human) Q9UQB9

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Entrez Gene ID: (Human) 6790, (Human) 9212, (Human) 6795

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity transferase activity protein kinase binding ubiquitin protein ligase binding histone kinase activity (H3-S10 specific) protein heterodimerization activity protein serine kinase activity molecular function activator activity kinase binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter mitotic cell cycle mitotic cytokinesis negative regulation of B cell apoptotic process spindle organization mitotic spindle organization positive regulation of microtubule depolymerization positive regulation of telomere maintenance regulation of cytokinesis negative regulation of cytokinesis positive regulation of cytokinesis regulation of microtubule-based process protein localization to kinetochore cellular response to UV cleavage furrow formation post-translational protein modification cell cycle G2/M phase transition mitotic cytokinesis checkpoint negative regulation of innate immune response mitotic spindle midzone assembly cell division attachment of mitotic spindle microtubules to kinetochore regulation of chromosome segregation midbody abscission positive regulation of mitotic sister chromatid segregation positive regulation of cell cycle process positive regulation of mitotic cell cycle spindle assembly checkpoint mitotic spindle assembly repair of mitotic kinetochore microtubule attachment defect cGAS/STING signaling pathway negative regulation of cGAS/STING signaling pathway regulation of signal transduction by p53 class mediator positive regulation of mitotic sister chromatid separation positive regulation of attachment of mitotic spindle microtubules to kinetochore positive regulation of mitotic cytokinesis positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore mitotic sister chromatid biorientation G2/M transition of mitotic cell cycle meiotic spindle organization microtubule cytoskeleton organization chromatin remodeling protein phosphorylation apoptotic process spindle assembly involved in female meiosis I centrosome cycle mitotic centrosome separation response to wounding anterior/posterior axis specification regulation of G2/M transition of mitotic cell cycle negative regulation of gene expression peptidyl-serine phosphorylation cell projection organization regulation of protein stability negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of mitotic nuclear division positive regulation of mitotic cell cycle regulation of centrosome cycle protein autophosphorylation meiotic cell cycle centrosome localization cilium disassembly protein localization to centrosome positive regulation of mitochondrial fission liver regeneration positive regulation of oocyte maturation neuron projection extension attachment of spindle microtubules to kinetochore
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