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Phospho-CD247 (CD3 zeta) (Lys111) Monoclonal Antibody (EM-55), Alexa Fluor™ 488
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FIGURE: 1 / 1
Phospho-CD247 (CD3 zeta) (Lys111) Antibody (MA5-38688) in Flow
Flow cytometry of CD247 (CD3 zeta) in pervanadate treated Jurkat cells (and mouse IgG1 isotype control). Samples were incubated with CD247 (CD3 zeta) monoclonal antibody (Product # MA5-38688) followed by Alexa Fluor 488 at a dilution of 5 µg/mL (red-filled and black-dashed).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-38688
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
EM-55
Immunogen
BP1 (phosphorylated Tyr111/123) - KLH
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes phosphorylated intracellular tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Target Information
CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, zeta polypeptide; CD3-epsilon; CD3-zeta/eta; CD3e antigen; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; component of the TCR/CD3 complex; FLJ18683; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 eta chain; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; T3E; TCR zeta; TCR zeta chain; TCRE; tissue factor-targeting CAR1 dimer; tissue factor-targeting CAR1 monomer; unnamed protein product
Gene Aliases: 4930549J05Rik; A430104F18Rik; Cd3; Cd3-eta; CD3-ZETA; CD3H; CD3Q; CD3Z; CD3ZETA; IMD25; T3Z; Tcrk; TCRZ
UniProt ID: (Human) P20963, (Mouse) P24161
Entrez Gene ID: (Human) 919, (Mouse) 12503
adaptive immune response
cell surface receptor signaling pathway
Fc-gamma receptor signaling pathway
gamma-delta T cell activation
alpha-beta T cell activation
T cell receptor signaling pathway
protein oligomerization
protein-containing complex assembly
positive regulation of protein localization to cell surface
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