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Invitrogen
RORB Recombinant Rabbit Monoclonal Antibody (7J9T7)
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FIGURE: 1 / 1
RORB Antibody (MA5-42735) in WB
Western blot analysis of RORB in extracts of various cell lines. Samples were incubated with RORB Monoclonal antibody (Product # MA5-42735) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
MA5-42735
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7J9T7
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RORβ (Q92753)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Amount
150 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: PC-12, C6, SH-SY5Y, Y79, Mouse eye.
Immunogen sequence: MCENQLKTKA DATAQIEVIP CKICGDKSSG IHYGVITCEG CKGFFRRSQQ NNASYSCPRQ RNCLIDRTNR NRCQHCRLQK CLALGMSRDA VKFGRMSKKQ
Target Information
Retinoid-related orphan receptor beta (ROR beta), a NR1 thyroid hormone receptor, affects the development and functioning of the central nervous system, including sensory input integration and circadian timing. Disruption of ROR beta in mice results in juvenile ataxia, retinal degeneration, circadian activity abnormalities, and delayed onset of male fertility. ROR beta binds to the monomeric response elements containing the sequence AnnTAGGTCA as a monomer. An alternatively spliced isoform, ROR beta2, has been isolated from rats, and the expression of ROR beta2 is confined to the pineal gland and retina. ROR beta2 shares common DNA- and putative ligand-binding domains with wild-type ROR beta but is characterized by a different amino-terminal domain. ROR beta expression has been documented in various regions of the mouse brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nuclear receptor ROR-beta; Nuclear receptor RZR-beta; Nuclear receptor subfamily 1 group F member 2; RAR-related orphan receptor beta; retinoic acid-binding receptor beta; retinoid Z receptor beta; retinoid-related orphan receptor beta; Retinoid-related orphan receptor-beta; transcription factor; unnamed protein product
Gene Aliases: bA133M9.1; EIG15; NR1F2; ROR-BETA; RORB; RORbeta; RZR-BETA; RZRB
UniProt ID: (Human) Q92753, (Mouse) Q8R1B8
Entrez Gene ID: (Human) 6096, (Rat) 309288, (Mouse) 225998
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
melatonin receptor activity
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
steroid hormone receptor activity
transcription factor binding
C4 zinc finger nuclear receptor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
G-protein coupled receptor signaling pathway
visual perception
regulation of gene expression
intracellular receptor signaling pathway
amacrine cell differentiation
eye photoreceptor cell development
regulation of circadian rhythm
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
retinal rod cell development
retinal cone cell development
rhythmic process
retina development in camera-type eye
retinal rod cell differentiation
cellular response to retinoic acid
transcription, DNA-templated
brain development
circadian rhythm
response to hormone
steroid hormone mediated signaling pathway
multicellular organism development
response to stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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