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Invitrogen
PKN2 Recombinant Rabbit Monoclonal Antibody (JE62-44)
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FIGURE: 1 / 3
PKN2 Antibody (MA5-44818) in ICC/IF
Immunocytochemistry analysis of PKN2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Samples were incubated in PKN2 Monoclonal antibody (Product # MA5-44818) using a dilution of 1:50 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG secondary antibody at a dilution of 1:1,000. The nuclear counter stain is DAPI (blue).
Product Details
MA5-44818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE62-44
Immunogen
Recombinant protein within human PKN2 aa 885-984/984.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PKN2 (PRK2), a close relative of the PKCs, is a regulator of mitosis and exit from cytokinesis. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiolipin-activated protein kinase Pak2; p140 kinase; PKN gamma; protease-activated kinase 2; Protein kinase C-like 2; Protein-kinase C-related kinase 2; RP5-905H16.1; Serine/threonine-protein kinase N2; unnamed protein product
Gene Aliases: Pak-2; PAK2; PKN2; PRK2; PRKCL2; PRO2042; STK7
UniProt ID: (Human) Q16513
Entrez Gene ID: (Human) 5586, (Rat) 207122
nucleotide binding
RNA binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
small GTPase binding
histone deacetylase binding
cadherin binding
RNA polymerase binding
protein serine kinase activity
poly(A) RNA binding
non-receptor serine/threonine protein kinase
protein phosphorylation
apoptotic process
cell adhesion
signal transduction
epithelial cell migration
cell projection organization
positive regulation of cytokinesis
intracellular signal transduction
apical junction assembly
positive regulation of viral genome replication
positive regulation of mitotic cell cycle
cell division
vascular endothelial cell response to laminar fluid shear stress
regulation of cell motility
transcription, DNA-templated
regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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