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Invitrogen
HSP60 Monoclonal Antibody (LK1), PerCP
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FIGURE: 1 / 4
HSP60 Antibody (MA5-45111) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol at -20°C for 10 minutes. Samples were incubated with Hsp60 monoclonal antibody (Product # MA5-45111) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45111
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Fruit fly,
Guinea pig,
Hamster,
Human,
Insect,
Mouse,
Non-human primate,
Sheep,
Plant,
Pig,
Rabbit,
Rat,
Shrew,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LK1
Immunogen
Recombinant human HSP60
Conjugate
PerCP
PerCP
PerCP
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.05 µg/mL of MA5-45111 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody. |Detects approximately 60kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60-kDa heat shock protein, mitochondrial; a stress-inducible molecular chaperone which participates in protein folding; CG12101-PA; CG12101-PB; chaperonin; chaperonin (HSP60); Chaperonin 60; CPN60; epididymis secretory sperm binding protein; heat shock 60 kDa protein 1; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_1; heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_2; heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_4; Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 60 kDa; heat shock protein 65; heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; Hsp 60; HSP-60; HSP-65; hsp60; Hsp60A-PA; Hsp60A-PB; Hsp60s1; lethal(1)10Ac; mitochondrial heat shock 60 kDa protein 1; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; unnamed protein product
Gene Aliases: 12; 60kDa; BP5; CG12101; chaperonin; CPN60; d-hsp60; Dm10A; Dmel\CG12101; Dmel_CG12101; DmHsp60; G62; GROEL; HLD4; Hsp 60; HSP-60; HSP-65; HSP60; HSP60A; Hsp64; HSP65; Hspd1-30p; HuCHA60; I79_011398; IEF16; l(1)10Ac; l(1)BP5; l(1)dp025; l(1)G8; l(1)HM21; l(1)L12; Mmp-P1; SPG13; SSP 7506; XELAEV_18045048mg
ATP binding
isomerase activity
lipopolysaccharide binding
p53 binding
double-stranded RNA binding
protein binding
high-density lipoprotein particle binding
ubiquitin protein ligase binding
apolipoprotein A-I binding
chaperone binding
DNA replication origin binding
single-stranded DNA binding
RNA binding
ATPase activity
enzyme binding
apolipoprotein binding
cysteine-type endopeptidase activator activity
ATP-dependent protein folding chaperone
unfolded protein binding
macromolecular complex binding
modification-dependent protein binding
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
apoptotic mitochondrial changes
response to cold
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
mitochondrial unfolded protein response
protein refolding
B cell proliferation
T cell activation
positive regulation of macrophage activation
negative regulation of apoptotic process
protein import into mitochondrial intermembrane space
isotype switching to IgG isotypes
protein stabilization
positive regulation of T cell activation
interaction with symbiont
cellular response to interleukin-7
response to hypoxia
positive regulation of cytokine production
response to ischemia
response to unfolded protein
response to xenobiotic stimulus
response to activity
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
response to ATP
cellular response to heat
B cell activation
response to cocaine
response to hydrogen peroxide
negative regulation of neuron apoptotic process
response to estrogen
adhesion of symbiont to host
response to glucocorticoid
negative regulation of apoptotic process in bone marrow
negative regulation of execution phase of apoptosis
positive regulation of execution phase of apoptosis
negative regulation of reactive oxygen species biosynthetic process
response to resveratrol
'de novo' protein folding
chaperone-mediated protein complex assembly
protein maturation
protein targeting to mitochondrion
mitochondrion organization
response to heat
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