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Invitrogen

CaMKII alpha/beta Monoclonal Antibody (22B1), APC

View all (6) CaMKII alpha/beta antibodies

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Product Details

MA5-45146

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100-1:5,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

22B1

Immunogen

Synthetic peptide

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931600

Product Specific Information

1 µg/mL was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta ).

Target Information

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha CaM kinase II; alpha-CaMKII; alternatively spliced; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; Calcium, calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II alpha subunit; CaM kinase II beta subunit; CaM kinase II subunit alpha; CaM kinase II subunit beta; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; caMK-II subunit beta; proline rich calmodulin-dependent protein kinase; protein kinase homolog; unnamed protein product

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Gene Aliases: CAM2; CAMK2; CAMK2A; CAMK2B; Camk2d; CAMKA; CAMKB; CaMKII; CaMKIIalpha; CaMKIIbeta; CaMKIINalpha; Ck2b; KIAA0968; mKIAA0968; MRD53; MRD54; MRT63; PK2CDD; PKCCD; R74975

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UniProt ID: (Human) Q9UQM7, (Human) Q13554, (Mouse) P11798, (Rat) P11275, (Rat) P08413, (Mouse) P28652

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Entrez Gene ID: (Human) 815, (Human) 816, (Mouse) 12322, (Rat) 25400, (Rat) 24245, (Mouse) 12323

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity calmodulin-dependent protein kinase activity protein binding calmodulin binding ATP binding kinase activity transferase activity glutamate receptor binding identical protein binding protein homodimerization activity metal ion binding protein serine kinase activity actin binding transferase activity, transferring phosphorus-containing groups GTPase activating protein binding phospholipase binding non-receptor serine/threonine protein kinase
Process(es)
G1/S transition of mitotic cell cycle response to ischemia protein phosphorylation calcium ion transport JAK-STAT cascade positive regulation of cardiac muscle cell apoptotic process cellular response to interferon-beta angiotensin-activated signaling pathway positive regulation of JAK-STAT cascade regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity negative regulation of hydrolase activity positive regulation of calcium ion transport long-term synaptic potentiation dendritic spine development cellular response to interferon-gamma regulation of mitochondrial membrane permeability involved in apoptotic process regulation of protein localization to plasma membrane peptidyl-threonine autophosphorylation regulation of endocannabinoid signaling pathway regulation of neuron migration signal transduction nervous system development positive regulation of neuron projection development regulation of skeletal muscle adaptation cell differentiation protein autophosphorylation regulation of long-term neuronal synaptic plasticity regulation of synapse structural plasticity regulation of calcium ion transport regulation of dendritic spine development positive regulation of dendritic spine morphogenesis positive regulation of synapse maturation phosphorylation peptidyl-serine phosphorylation ionotropic glutamate receptor signaling pathway inhibitory G-protein coupled receptor phosphorylation regulation of synaptic transmission, cholinergic positive regulation of phospholipase A2 activity response to cadmium ion cell projection morphogenesis neuromuscular process controlling balance activation of meiosis involved in egg activation positive regulation of apoptotic signaling pathway
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