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Invitrogen

HO-1 Monoclonal Antibody (1F12-A6), APC

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HO-1 Monoclonal Antibody (1F12-A6), APC

Product Details

MA5-45152

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1F12-A6

Immunogen

Human HO-1 synthetic peptide, amino acids 1-30
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931606

Product Specific Information

1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects 32kDa. Does not cross-react with HO-2.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: heat shock protein 32; heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HMOX1; HO-1; HSP32; P32 protein; unnamed protein product

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Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Pig) P32394, (Human) P09601, (Bovine) Q5E9F2, (Rat) P06762, (Mouse) P14901

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Entrez Gene ID: (Pig) 445512, (Rabbit) 100008919, (Human) 3162, (Dog) 442987, (Bovine) 513221, (Guinea pig) 100715748, (Rat) 24451, (Chinese hamster) 100756872, (Mouse) 15368

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Function(s)
heme oxygenase (decyclizing) activity heme binding identical protein binding protein homodimerization activity metal ion binding structural molecule activity protein binding oxidoreductase activity enzyme binding phospholipase D activity signal transducer activity oxygenase
Process(es)
wound healing involved in inflammatory response heme oxidation cellular iron ion homeostasis apoptotic process response to oxidative stress erythrocyte homeostasis regulation of transcription from RNA polymerase II promoter in response to iron response to nicotine heme catabolic process heme metabolic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation by host of viral transcription positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of viral life cycle angiogenesis endothelial cell proliferation negative regulation of leukocyte migration regulation of transcription from RNA polymerase II promoter smooth muscle hyperplasia macroautophagy positive regulation of macroautophagy negative regulation of macroautophagy positive regulation of chemokine production low-density lipoprotein particle clearance cellular response to heat intracellular signal transduction response to hydrogen peroxide regulation of angiogenesis positive regulation of angiogenesis multicellular organismal iron ion homeostasis cellular response to cisplatin positive regulation of cell migration involved in sprouting angiogenesis negative regulation of ferroptosis negative regulation of cytokine production involved in inflammatory response positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis epithelial cell apoptotic process positive regulation of epithelial cell apoptotic process response to hypoxia phospholipid metabolic process small GTPase mediated signal transduction regulation of blood pressure cell death negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process cellular response to nutrient negative regulation of mast cell cytokine production negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization cellular response to hypoxia oxidation-reduction process
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