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Invitrogen
CD107a (LAMP-1) Monoclonal Antibody (Ly1C6), APC
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FIGURE: 1 / 1
CD107a (LAMP-1) Antibody (MA5-45166) in WB
Western Blot analysis of Rat liver microsome lysate showing detection of LAMP1 protein. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with LAMP1 monoclonal antibody (Product # MA5-45166) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Details
MA5-45166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Ly1C6
Immunogen
Rat liver lysosomal membrane preparations
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.|Detects approximately 120kDa.
Target Information
LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer's Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; CD107a; Lamp I; LAMP-1; LGP-120; LGP-A; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lysosome-associated membrane protein 1; membrane glycoprotein; P2B; unnamed protein product
Gene Aliases: AI196048; CD107a; I79_011073; LAMP-1; LAMP1; LAMPA; LGP-A; LGP120; LGPA
UniProt ID: (Human) P11279, (Chinese hamster) P49129, (Mouse) P11438, (Rat) P14562
Entrez Gene ID: (Human) 3916, (Chinese hamster) 100689406, (Mouse) 16783, (Rat) 25328
lysosomal lumen acidification
lysosomal lumen pH elevation
positive regulation of natural killer cell degranulation
positive regulation of natural killer cell mediated cytotoxicity
viral entry into host cell
protein stabilization
establishment of protein localization to organelle
Golgi to lysosome transport
granzyme-mediated programmed cell death signaling pathway
regulation of organelle transport along microtubule
hydrogen ion transmembrane transport
spermatogenesis
granzyme-mediated apoptotic signaling pathway
autophagic cell death
autophagy
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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