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Invitrogen
CD107b (LAMP-2) Monoclonal Antibody (GL2A7), APC
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FIGURE: 1 / 1
CD107b (LAMP-2) Antibody (MA5-45169) in WB
Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein. Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 µg). Lane 3: Mouse 3T3NIH lysate (10 µg). Blocking: 5% milk + TBST for 1 hour at RT. Samples were incubated with LAMP2 monoclonal antibody (Product # MA5-45169) at 1:500 for 1 hour at RT, followed by HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GL2A7
Immunogen
Purified preparation of mouse liver lysosomal membranes
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45169 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100-110kDa.
Target Information
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD107 antigen-like family member B; Igp96; LAMP-2 Isoform 2A; LAMP-2 Variant A; LAMP-2C; lysosomal associated membrane protein 2; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosomal membrane glycoprotein-type B; RP23-193O17.2; unnamed protein product
Gene Aliases: CD107b; DND; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; LAMPB; LGP-96; LGP-B; LGP110; Mac3
UniProt ID: (Human) P13473, (Mouse) P17047
Entrez Gene ID: (Rabbit) 100350946, (Human) 3920, (Mouse) 16784
protein targeting
autophagy
lysosomal lumen acidification
cellular response to starvation
protein import
protein catabolic process
negative regulation of protein complex assembly
regulation of protein stability
protein stabilization
chaperone-mediated autophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
autophagosome maturation
positive regulation of ferroptosis
negative regulation of NLRP3 inflammasome complex assembly
lysosomal protein catabolic process
lysosomal lumen pH elevation
muscle cell cellular homeostasis
hydrogen ion transmembrane transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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